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1. Retracted: "CITRX thioredoxin interacts with the tomato Cf-9 resistance protein and negatively regulates defence".

Author

Rivas S, Rougon-Cardoso A, Smoker M, Schauser L, Yoshioka H, and Jones JD

Abstract

The authors regret to announce they would like to withdraw this paper, for two main reasons: Since the paper was published, it has become clear that the thioredoxin that interacts in yeast 2-hybrid with the Cf-9 C-terminus is in fact localized in the chloroplast, rendering a role in Cf-9 signalling unlikely. Close scrutiny of the figures suggests several duplications. - In Fig 3A, the Anti-MBP band in lane 4 closely resembles the antiMBP band in Fig 3B lane 1, though slightly rotated. - In Fig 6A, the leaf disc in the panel labelled TRV:00, -Avr9, 30 min looks identical to the leaf disc in Fig S5, panel labelled Cf2 TRV:CITRX, -Avr2, 1 h. - In Fig 6C, multiple bands appear duplicated. For example, GlucA, TRV:00, -Avr9, 0 h duplicated with 6 h; and GlucB, TRV:00, +Avr9, 0 h duplicated with Hin1, TRV:00, +Avr9, 0 h. Source data for these figures are not available. All the authors agree that this paper should be withdrawn from the scientific literature., (© 2019 The Authors.)

Published
2019
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2. CITRX thioredoxin interacts with the tomato Cf-9 resistance protein and negatively regulates defence.

Author

Rivas S, Rougon-Cardoso A, Smoker M, Schauser L, Yoshioka H, and Jones JD

Subjects
Amino Acid Sequence, Base Sequence, Enzyme Activation, Gene Expression Regulation, Plant, Gene Silencing, Hydrogen Peroxide metabolism, Immunity, Innate genetics, Solanum lycopersicum genetics, Molecular Sequence Data, Oxidants metabolism, Phylogeny, Plant Leaves anatomy & histology, Plant Leaves metabolism, Plant Proteins classification, Plant Proteins genetics, Plant Viruses genetics, Plant Viruses metabolism, Protein Kinases metabolism, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins metabolism, Thioredoxins classification, Thioredoxins genetics, Nicotiana genetics, Nicotiana metabolism, Two-Hybrid System Techniques, Immunity, Innate physiology, Solanum lycopersicum chemistry, Membrane Glycoproteins metabolism, Plant Proteins metabolism, Thioredoxins metabolism
Abstract

To identify proteins involved in tomato Cf-9 resistance protein function, a yeast two-hybrid screen was undertaken using the cytoplasmic C-terminus of Cf-9 as bait. A thioredoxin-homologous clone, interacting specifically with Cf-9, was identified and called CITRX (Cf-9-interacting thioredoxin). Virus-induced gene silencing (VIGS) of CITRX resulted in an accelerated Cf-9/Avr9-triggered hypersensitive response in both tomato and Nicotiana benthamiana, accompanied by enhanced accumulation of reactive oxygen species, alteration of protein kinase activity and induction of defence-related genes. VIGS of CITRX also conferred increased resistance to the fungal pathogen Cladosporium fulvum in the otherwise susceptible Cf0 tomato. CITRX acts as a negative regulator of the cell death and defence responses induced through Cf-9, but not Cf-2. Recognition of the Cf-9 C-terminus by CITRX is necessary and sufficient for this negative regulation. This is the first study that implicates thioredoxin activity in the regulation of plant disease resistance.

Published
2004
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