1. Mapping cortical mesoscopic networks of single spiking cortical or sub-cortical neurons.
- Author
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Xiao D, Vanni MP, Mitelut CC, Chan AW, LeDue JM, Xie Y, Chen AC, Swindale NV, and Murphy TH
- Subjects
- Anesthesia, Animals, Brain Mapping, Calcium physiology, Calcium Signaling physiology, Cerebral Cortex anatomy & histology, Electrodes, Implanted, Male, Mice, Mice, Transgenic, Molecular Probes chemistry, Molecular Probes genetics, Nerve Net anatomy & histology, Neurons cytology, Optical Imaging methods, Stereotaxic Techniques, Thalamus anatomy & histology, Wakefulness physiology, Action Potentials physiology, Cerebral Cortex physiology, Nerve Net physiology, Neurons physiology, Thalamus physiology
- Abstract
Understanding the basis of brain function requires knowledge of cortical operations over wide-spatial scales, but also within the context of single neurons. In vivo, wide-field GCaMP imaging and sub-cortical/cortical cellular electrophysiology were used in mice to investigate relationships between spontaneous single neuron spiking and mesoscopic cortical activity. We make use of a rich set of cortical activity motifs that are present in spontaneous activity in anesthetized and awake animals. A mesoscale spike-triggered averaging procedure allowed the identification of motifs that are preferentially linked to individual spiking neurons by employing genetically targeted indicators of neuronal activity. Thalamic neurons predicted and reported specific cycles of wide-scale cortical inhibition/excitation. In contrast, spike-triggered maps derived from single cortical neurons yielded spatio-temporal maps expected for regional cortical consensus function. This approach can define network relationships between any point source of neuronal spiking and mesoscale cortical maps.
- Published
- 2017
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