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1. Concerted conformational dynamics and water movements in the ghrelin G protein-coupled receptor

Author

Maxime Louet, Marina Casiraghi, Marjorie Damian, Mauricio GS Costa, Pedro Renault, Antoniel AS Gomes, Paulo R Batista, Céline M'Kadmi, Sophie Mary, Sonia Cantel, Severine Denoyelle, Khoubaib Ben Haj Salah, David Perahia, Paulo M Bisch, Jean-Alain Fehrentz, Laurent J Catoire, Nicolas Floquet, and Jean-Louis Banères

Subjects
GPCR, hydration, signaling, Medicine, Science, Biology (General), QH301-705.5
Abstract

There is increasing support for water molecules playing a role in signal propagation through G protein-coupled receptors (GPCRs). However, exploration of the hydration features of GPCRs is still in its infancy. Here, we combined site-specific labeling with unnatural amino acids to molecular dynamics to delineate how local hydration of the ghrelin receptor growth hormone secretagogue receptor (GHSR) is rearranged upon activation. We found that GHSR is characterized by a specific hydration pattern that is selectively remodeled by pharmacologically distinct ligands and by the lipid environment. This process is directly related to the concerted movements of the transmembrane domains of the receptor. These results demonstrate that the conformational dynamics of GHSR are tightly coupled to the movements of internal water molecules, further enhancing our understanding of the molecular bases of GPCR-mediated signaling.

Published
2021
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2. Concerted conformational dynamics and water movements in the ghrelin G protein-coupled receptor

Author

Laurent J. Catoire, Sonia Cantel, Marjorie Damian, Jean-Alain Fehrentz, Maxime Louet, Antoniel As Gomes, Jean-Louis Banères, Sophie Mary, Paulo R. Batista, David Perahia, Paulo Mascarello Bisch, Mauricio Gs Costa, Khoubaib Ben Haj Salah, Céline M'Kadmi, Marina Casiraghi, Pedro Renault, Severine Denoyelle, Nicolas Floquet, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de biologie physico-chimique des protéines membranaires (LBPC-PM (UMR_7099)), Institut de biologie physico-chimique (IBPC (FR_550)), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Department of Molecular and Cellular Physiology [Stanford], Stanford Medicine, Stanford University-Stanford University, Laboratoire de biologie et pharmacologie appliquée (LBPA), Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Ecole Normale Supérieure Paris-Saclay (ENS Paris Saclay), Universidade Federal do Rio de Janeiro (UFRJ), This work was supported by CNRS, Université de Montpellier, Agence Nationale de la Recherche (ANR-17-CE11-0011, ANR-17-CE11-22, ANR-17-CE18-0022), EpiGenMed Labex (post-doctoral fellowship to KBH) and DYNAMO Labex (post-doctoral fellowship to MC). This programreceived funding from the European Union’s Horizon 2020 research and innovation programmeunder the Marie Sklodowska-Curie grant agreement n˚ 799376. Mass spectrometry analyses wereperformed on the instruments located in the IBMM platform of instrumentation, Laboratoire deMesures Physiques (LMP) of Université de Montpellier. We thank GENCI (Grand EquipementNational de Calcul Intensif), CINES (Centre Informatique National de l’Enseignement Supérieur), and IDRIS (Institut du développement et des ressources en informatique scientifique) for computational, ANR-17-CE11-0011,allosig,allostérie, dynamique conformationnelle et signalisation via les RCPG(2017), ANR-17-CE11-0022,GPCteR,Mécanismes moléculaires des régions C-terminales désordonnées et fonctionnelles des RCPG et impact sur les voies de la signalisation cellulaire dépendantes de l'arrestine(2017), and Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)

Subjects
QH301-705.5, Science, Growth hormone secretagogue receptor, Chemical biology, chemical biology, 010402 general chemistry, Ligands, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, Receptors, G-Protein-Coupled, 03 medical and health sciences, Molecular dynamics, GPCR, Biochemistry and Chemical Biology, biochemistry, Humans, Biology (General), Receptor, Receptors, Ghrelin, 030304 developmental biology, G protein-coupled receptor, chemistry.chemical_classification, 0303 health sciences, General Immunology and Microbiology, [SDV.BA]Life Sciences [q-bio]/Animal biology, General Neuroscience, digestive, oral, and skin physiology, E. coli, General Medicine, Ghrelin, 0104 chemical sciences, Amino acid, [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, Transmembrane domain, chemistry, Biophysics, Medicine, signaling, hydration, Research Article, Human, Signal Transduction
Abstract

International audience; There is increasing support for water molecules playing a role in signal propagation through G protein-coupled receptors (GPCRs). However, exploration of the hydration features of GPCRs is still in its infancy. Here, we combined site-specific labeling with unnatural amino acids to molecular dynamics to delineate how local hydration of the ghrelin receptor growth hormone secretagogue receptor (GHSR) is rearranged upon activation. We found that GHSR is characterized by a specific hydration pattern that is selectively remodeled by pharmacologically distinct ligands and by the lipid environment. This process is directly related to the concerted movements of the transmembrane domains of the receptor. These results demonstrate that the conformational dynamics of GHSR are tightly coupled to the movements of internal water molecules, further enhancing our understanding of the molecular bases of GPCR-mediated signaling.

Published
2021

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