1. Zebrafish Posterior Lateral Line primordium migration requires interactions between a superficial sheath of motile cells and the skin
- Author
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Naveen Natesh, Hari Shroff, Ajay B. Chitnis, Damian Dalle Nogare, and Harshad D. Vishwasrao
- Subjects
0301 basic medicine ,Embryo, Nonmammalian ,animal structures ,QH301-705.5 ,Science ,Population ,Embryonic Development ,Fibroblast growth factor ,General Biochemistry, Genetics and Molecular Biology ,Collective migration ,lateral line primordium ,03 medical and health sciences ,0302 clinical medicine ,Cell Movement ,Animals ,Fgf ,Primordium ,Biology (General) ,education ,Zebrafish ,education.field_of_study ,Matrigel ,collective cell migration ,General Immunology and Microbiology ,biology ,Chemistry ,General Neuroscience ,Gene Expression Regulation, Developmental ,Cell Biology ,General Medicine ,Zebrafish Proteins ,biology.organism_classification ,Lateral Line System ,Cell biology ,030104 developmental biology ,lamellipodia ,embryonic structures ,Medicine ,Lamellipodium ,Developmental biology ,030217 neurology & neurosurgery ,Research Article ,Developmental Biology - Abstract
The Zebrafish Posterior Lateral Line primordium migrates in a channel between the skin and somites. Its migration depends on the coordinated movement of its mesenchymal-like leading cells and trailing cells, which form epithelial rosettes, or protoneuromasts. We describe a superficial population of flat primordium cells that wrap around deeper epithelialized cells and extend polarized lamellipodia to migrate apposed to the overlying skin. Polarization of lamellipodia extended by both superficial and deeper protoneuromast-forming cells depends on Fgf signaling. Removal of the overlying skin has similar effects on superficial and deep cells: lamellipodia are lost, blebs appear instead, and collective migration fails. When skinned embryos are embedded in Matrigel, basal and superficial lamellipodia are recovered; however, only the directionality of basal protrusions is recovered, and migration is not rescued. These observations support a key role played by superficial primordium cells and the skin in directed migration of the Posterior Lateral Line primordium.
- Published
- 2020