1. Mitochondrial D-loop 3' (CA)n repeat polymorphism: optimization of analysis and population data
- Author
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Ines Plate, Evgeni K. Ginter, D. Krause, Matthias Michael, Victor A. Spitsyn, and Reinhard Szibor
- Subjects
Mitochondrial DNA ,Clinical Biochemistry ,Population ,Black People ,Biology ,Biochemistry ,DNA, Mitochondrial ,White People ,Analytical Chemistry ,D-loop ,Polymorphism (computer science) ,Humans ,Allele ,education ,Dinucleotide Repeats ,Gene ,Alleles ,mtDNA control region ,Genetics ,education.field_of_study ,Polymorphism, Genetic ,Forensic Medicine ,Molecular biology ,Europe ,Africa ,Primer (molecular biology) - Abstract
We report a dinucleotide repeat polymorphism in the 3′ area of the mitochondrial control region. The fragments obtained using a new primer set could be reliably separated by polyacrylamide gel electrophoresis (PAGE) using nondenaturing gels. A total of five alleles [(CA)3 to (CA)7] were detected on silver-stained gels. The 90 bp product corresponds to allele 5. Samples from one African and three European populations were characterized. Significant differences could be demonstrated as to the incidence of single alleles and allele distributions in different populations. These differences were found between the three European and one African Bantu population. For specific forensic questions the mitochondrial CA repeat is well suited. Gene diversities in populations of Germany, Hungary, the Russian Federation and Cameroon were 0.36, 0.40, 0.34, 0.52, respectively.
- Published
- 1998