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Your search keyword '"Ju, Huangxian"' showing total 31 results
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31 results on '"Ju, Huangxian"'

27. Nanogold-Enriched Carbon Nanohorn Label for Sensitive Electrochemical Detection of Biomarker on a Disposable Immunosensor.

Author

Zhao , Changrong, Lin, Dajie, Wu, Jie, Ding, Lin, Ju, Huangxian, and Yan, Feng

Abstract

A novel nanogold-enriched carbon nanohorn structure (nanoAu/CNH) was designed as a trace tag for highly efficient electrochemical detection of tumor marker using a disposable immunosensor. The nanoAu/CNH was synthesized by one-pot in situ growth of nanogold on carboxylated single-walled CNH. The labeling of signal antibody was performed via the inherent interaction between protein and nanogold. The disposable immunosensor was constructed by covalently cross-linking capture antibody on chitosan modified screen-printed carbon electrode. With a sandwich format, the nanoAu/CNH labeled antibody was conjugated on the immunosensor for electrochemical measurement of tumor marker by electrooxidizing the nanoAu at +1.3 V and then cathodic potential scan in 0.1 M HCl. Using α-fetoprotein as model target, the proposed immunoassay showed acceptable precision and wide linear range from 0.1 pg mL−1 to 1 ng mL−1 with a detection limit of 0.07 pg mL−1. The new nanoAu/CNH label as well as the disposable immunosensor showed possessed potential application in point-of-care testing. [ABSTRACT FROM AUTHOR]

Published
2013
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30. Simultaneous Electrochemiluminescence Detection of Anisodamine, Atropine, and Scopolamine in Flos daturaeby Capillary Electrophoresis Using β‐Cyclodextrin as Additive

Author

Li, Jianguo, Chun, Yuan, and Ju, Huangxian

Abstract

This work developed a simple and sensitive method for simultaneous determination of three effective ingredients, atropine, scopolamine and anisodamine, in Flos daturaebased on capillary electrophoresis coupled with electrochemiluminescence detection. β‐Cyclodextrin was used as an additive to the running buffer for obtaining the absolute separation. The proposed method displayed the linear ranges from 0.2 to 100, 0.2 to 100 and 20 to 200 μM for anisodamine, atropine and scopolamine with correlation coefficients more than 0.99, respectively. This method showed the relative standard deviations less than 4% and 6% for detection of migration time and peak height, respectively, and was suitable for the determination of these tropane alkaloids in plants and valuable in clinical and biochemical laboratories for quality control.

Published
2007
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31. Study on Electrochemiluminesce of Ru(bpy3)2+Immobilized in a Titania Sol‐Gel Membrane

Author

Zhuang, Yafeng and Ju, Huangxian

Abstract

The immobilization of tris(2,2′‐bipyridyl)ruthenium(II), Ru(bpy)32+, at a glassy carbon electrode was achieved by entrapping the Ru(bpy)32+in a vapor deposited titania sol‐gel membrane. The electrogenerated chemiluminescence (ECL) of the immobilized Ru(bpy)32+was studied. The Ru(bpy)32+modified electrode showed a fast ECL response to both oxalate and proline. The ECL intensity was linearly related to concentrations of oxalate and proline over the ranges from 20 to 700 μmol L−1and 20 to 600 μmol L−1, respectively. The detection limits for oxalate and proline at 3σ were 5.0 μmol L−1and 4.0 μmol L−1, respectively. This electrode possessed good precision and stability for oxalate and proline determinations. The electrogenerated chemiluminescence mechanism of proline system was discussed. This work provided a new way for the immobilization of Ru(bpy)32+and the application of titania sol‐gel membrane in electrogenerated chemiluminescence.

Published
2004
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