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Ferrocene‐derivatives have a well‐defined electrochemical behaviour, which can be changed upon binding to other molecules (including nonelectroactive species), as the conjugate presents a slower diffusion to the electrode surface. Therefore, the binding event can be easily detected by monitoring the decrease in the current intensity of the ferrocene‐derivative oxidation peak. An electrochemical probe composed of ferrocenecarboxylic acid conjugated with Sambucus nigra agglutinin was prepared and used to selectively detect the STn antigen, a pan‐carcinoma glycobiomarker. The probe showed high selectivity towards the STn antigen present in glycoproteins and demonstrated effectiveness in the analysis of serum samples from carcinoma patients. [ABSTRACT FROM AUTHOR]

A Phaseolus vulgaris leukoagglutinin (PHA−L) impedimetric biosensor was developed for the selective detection of cancer‐associated N‐glycans presenting increased β1→6 GlcNAc branching. The increase in the biosensor's impedance after sample incubation was indicative of lectin recognition and complex formation between PHA−L and N‐glycans with increased β1→6 GlcNAc branching, present in serum glycoproteins. Bovine thyroglobulin was used as a model glycoprotein and a linear correlation was found between glycoprotein concentration and analytical signal from 0.05 to 2.0 mg ml−1. The biosensor was validated by analyzing serum samples from patients with diverse types of carcinomas. [ABSTRACT FROM AUTHOR]