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Your search keyword '"Fang, Yuzhi"' showing total 25 results
Start Over Author "Fang, Yuzhi" Journal electroanalysis
25 results on '"Fang, Yuzhi"'

2. The Split Primer Ligation‐triggered 8‐17 DNAzyme Assisted Cascade Rolling Circle Amplification for High Specific Detection of Liver Cancer‐involved mRNAs: TK1 and c‐myc.

Author

Dai, Shiyan, Zhou, Yuting, Dai, Peiqing, Cheng, Guifang, He, Pingang, and Fang, Yuzhi

Subjects
CIRCLE, LIVER cancer, LIVER, DETECTION limit, DNA primers, CANCER cells
Abstract

Simultaneous detection of various intracellular biomarkers is promising for early diagnosis and treatment of cancer. Herein, we develop a novel method for high specific and ultrasensitive detection of liver cancer cell‐involved mRNAs: TK1 and c‐myc based on the split primer ligation‐triggered 8‐17 DNAzyme assisted cascade rolling circle amplification. Only two targets exist simultaneously, can trigger the rolling circle amplification to improve the accuracy and sensitivity. Meanwhile, an electrochemical molecular beacon, based on the host‐guest recognition between ferrocene groups and cucurbit urils [7] (CB[7]/Fc‐MB), is used to cause a "turn‐off" electrochemical signal which is decreased by disrupting its hairpin structure. Under the optimal conditions, the detection limit of TK1 and c‐myc mRNA is as low as 0.06 nM. Moreover, this method can be used to detect the TK1 and c‐myc mRNA in HepG2 cells and distinguish between cancer cells and their normal cells, proving that the method has the potential to detect the variation of biomarkers in vivo. [ABSTRACT FROM AUTHOR]

Published
2020
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24. Direct Electrochemistry and Electrocatalysis of Hemoglobin on Aligned Carbon Nanotubes Based Electrodes Modified with Au Nanoparticles and SiO2 Gel.

Author

Yang, Jing, Xu, Ying, He, Pingang, and Fang, Yuzhi

Subjects
ELECTROCHEMISTRY, ELECTROCHEMICAL analysis, HEMOGLOBINOMETRY, CARBON nanotubes, CHEMICAL vapor deposition, FIELD emission electron microscopy, SCANNING electron microscopy
Abstract

Here we report on the preparation and characterization of new electrodes based on aligned carbon nanotubes (ACNTs) for hemoglobin (Hb) electrochemistry and electrocatalysis. The ACNTs are obtained by a thermal chemical vapor deposition method under normal pressure. Then the electrodes are elaborated by first sputtering a thin Au film (thickness of 200 nm) onto the top of the ACNTs, and then removing the Au layer/ACNTs from the quartz substrate with the aide of hydrofluoric acid (HF) treatment. Field emission scanning electron microscopy (FESEM) demonstrates that after nitric acid (HNO3) treatment, the nanotubes of the removed Au layer are totally tip-opened, purified and organized in a perfect vertically aligned architecture. The final ACNTs electrode is obtained by attaching the Au layer of ACNTs onto a glassy carbon electrode. Then the electrode was modified to act as a matrix for hemoglobin (Hb) immobilization and as an electrode for Hb electroanalysis by the assistance of Au nanoparticles (AuNPs) and SiO2 gel. Due to the individual specific effects of AuNPs, SiO2 gel and ACNTs, the resulting SiO2/Hb-AuNPs/ACNTs electrode showed good direct electrochemistry of Hb with an apparent MichaelisMenten constant of 0.44 mM. The electrode showed an excellent electrocatalytic activity towards H2O2, possessing a linear range from 40 µM to 4 mM and the detection limit was 22 µM based on a signal to noise ratio of 3. [ABSTRACT FROM AUTHOR]

Published
2013
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25. Determination of Taurine in Lycium Barbarum L.and Other Foods by Capillary Electrophoresis with Electrochemical Detection

Author

Cao, Yuhua, Zhang, Xin, Chu, Qingcui, Fang, Yuzhi, and Ye, Jiannong

Abstract

A method based on capillary electrophoresis (CE) with electrochemical detection (ED) was developed for the determination of taurine in Lycium Barbarum L., LIPOVIYAN beverage and milk powder. The effects of some important factors such as the acidity of the running buffer, separation voltage, injection time, and applied potential to working electrode were investigated. Operated in a wall‐jet configuration, a 300 μm diameter carbon‐disk electrode was used as the working electrode, which exhibits good responses at +1.05 V (vs. SCE) for taurine. Excellent linearity was obtained in the concentration range from 5.0×10−4mol/L to 5.0×10−6mol/L. The detection limit (S/N=3) was 1.0×10−7mol/L. This proposed method has been successfully applied to analyze the actual samples with satisfactory assay results.

Published
2003
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