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A novel sensor for sensitive determination of hydroquinone (HQ) and catechol (CC) was proposed using a Fe3O4 microspheres-graphene oxide (Fe3O4-GO) composites modified glassy carbon electrode (GCE) which was further encapsulated with chitosan (Chit-Fe3O4-GO/GCE). The excellent electrocatalytic performance is probably related to the strong synergistic effect of the composites. The proposed sensor shows a linear response for HQ and CC in wide concentration ranges from 1.5 to 150 µM and 1 to 410 µM, with detection limits ( S/ N=3) of 20 and 250 nM, respectively. Additionally, the sensor exhibits good selectivity and stability in tap water samples. [ABSTRACT FROM AUTHOR]