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Introduction: Natural extracts are a suitable alternative in reducing the harmful effects of drugs and chemicals. Aim of the Work: evaluated the role of the spirulina in reducing pathological tissue lesions in the liver and kidneys of gentamicin-treated rats, as well as the relationship between these effects and antioxidant levels and other biochemical factors. Materials and Methods: In this study, 24 rats were evenly distributed into 4 groups, each group comprising 6 rats. Group 1 cocedered control, Group 2 administered 1000 mg/kg of spirulina, Group 3 was administered 100 mg/kg of gentamicin I M. Group 4 was subjected to the same dosages and treatment methods as G2 and G3, with a 5-day course of Gentamicin and a 12-day course of spirulina. Results: Gentamicin group recorded significantly increase in Alanine aminotransferase, Aspartate transaminase, Creatinine, and Urea concentrations. The group given spirulina following gentamicin treatment had lower levels of liver and kidney enzymes than the group given gentamicin alone and there was also an increase in the glutathione, as well as a drop in malondialdehyde levels. In the histopathological analysis, the Gentamicin-treated group exhibited coagulative necrosis also vacuolar degeneration in liver cells, along with central vein congestion. In the kidneys, this group displayed glomeruli atrophy, dilatation of the Bowman's space, the presence of hyaline casts in tubules of renal, with coagulative necrosis of epithelial cells lining renal tubule, when Gentamicin combined with Spirulina, the liver section revealed intact hepatocytes. In the kidney section of the same group, glomeruli remained intact, with only mild cell swelling observed in the epithelial cells lining the tubules of renal. Conclusion: spirulina has beneficial effects in the liver and kidneys of rats given gentamicin. [ABSTRACT FROM AUTHOR]

Introduction: Gentamicin (GM) is a potent bactericidal, broad-spectrum aminoglycoside. Selenium nanoparticles (SeNPs) are featured with improved antioxidant ability compared to other chemical forms of selenium with reducing the risk of selenium toxicity. Aim of the Work: The current work attempted to evaluate the possible protective contribution of selenium nanoparticles to gentamicin-induced toxicity in the testis of rat. Material and Methods: 36 adult male albino rats were included in the current research. Their age ranges between 3-5 months and their weight (180-220g). Rats were categorized into three groups. Group I: It was composed of 12 rats, that were divided into three equivalent subgroups; Subgroup IA: compromising 4 rats maintained a negative control and received nothing but food and water for 6 days; Subgroup IB: included 4 rats that received 0.5 mg/kg intraperitoneal (IP) saline for 6 successive days and Subgroup IC included 4 rats that received selenium nanoparticles 0.5 mg/kg intraperitoneal (IP) for 6 successive days. Group II: It included twelve adult male rats that received gentamycin 100 mg/kg IP for 6 successive days. Group III: It included twelve rats that received both gentamicin and selenium nanoparticles. IP selenium nanoparticles will be administered to rats 1hr after the gentamicin treatment at the same dose and duration mentioned before. The serum testosterone level was determined. Sections of testis underwent histological, biochemical, morphometric and statistical analysis. Results: Gentamycin induced a significant reduction in testosterone level and degeneration of the spermatogenic epithelial series with large areas of vacuolations, as well as thick and irregular basement membrane. Ki67 count was recorded. Injection of SeNPs enhanced the aforementioned aspects. Conclusion: GM resulted in histological as well as biochemical changes in the testes of adult male rats. Administration of SeNPs with GM attenuated these negative impacts which can be attributed to the antioxidant activity. [ABSTRACT FROM AUTHOR]