1. Epstein–Barr Virus MicroRNAs are Expressed in Patients with Chronic Lymphocytic Leukemia and Correlate with Overall Survival
- Author
-
Ferrajoli, Alessandra, Ivan, Cristina, Ciccone, Maria, Shimizu, Masayoshi, Kita, Yoshiaki, Ohtsuka, Masahisha, D'Abundo, Lucilla, Qiang, Jun, Lerner, Susan, Nouraee, Nazila, Rabe, Kari G, Rassenti, Laura Z, Van Roosbroeck, Katrien, Manning, John T, Yuan, Yuan, Zhang, Xinna, Shanafelt, Tait D, Wierda, William G, Sabbioni, Silvia, Tarrand, Jeffrey J, Estrov, Zeev, Radovich, Milan, Liang, Han, Negrini, Massimo, Kipps, Thomas J, Kay, Neil E, Keating, Michael, and Calin, George A
- Subjects
Biomedical and Clinical Sciences ,Oncology and Carcinogenesis ,Lymphoma ,Genetics ,Rare Diseases ,Clinical Research ,Cancer ,Biotechnology ,Hematology ,Aetiology ,2.1 Biological and endogenous factors ,Infection ,Disease-Free Survival ,Epstein-Barr Virus Nuclear Antigens ,Herpesvirus 4 ,Human ,Humans ,Leukemia ,Lymphocytic ,Chronic ,B-Cell ,MicroRNAs ,RNA ,Viral ,Tumor Cells ,Cultured ,Tumor Suppressor Protein p53 ,Viral Matrix Proteins ,Viral Proteins ,beta 2-Microglobulin ,miRNAs ,Epstein-Barr Virus ,Chronic lymphocytic leukemia ,BHRF1-1 ,Overall survival ,Epstein–Barr Virus ,Clinical Sciences ,Public Health and Health Services ,Clinical sciences ,Epidemiology - Abstract
Although numerous studies highlighted the role of Epstein-Barr Virus (EBV) in B-cell transformation, the involvement of EBV proteins or genome in the development of the most frequent adult leukemia, chronic lymphocytic leukemia (CLL), has not yet been defined. We hypothesized that EBV microRNAs contribute to progression of CLL and demonstrated the presence of EBV miRNAs in B-cells, in paraffin-embedded bone marrow biopsies and in the plasma of patients with CLL by using three different methods (small RNA-sequencing, quantitative reverse transcription PCR [q-RT-PCR] and miRNAs in situ hybridization [miRNA-ISH]). We found that EBV miRNA BHRF1-1 expression levels were significantly higher in the plasma of patients with CLL compared with healthy individuals (p
- Published
- 2015