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Cattle of polled phenotype is convenient for breeders, as it decreases the risk of animals being hurt and ensures safety of workers. We developed the system for editing cattle genome using CRISPR/Cas9 which will allow production of animals with polled phenotype genetically based on any cattle breed without changing its main phenotypic traits.

Beta-lactoglobulin is the main allergen of cow’s milk. Modern approaches to reducing the allergenicity of milk require significant costs for its fermentation. An alternative approach could be the creation of productive breeds with a knockout for the gene of this protein. This will allow, at no additional cost, to increase its safety for children who are breast-fed. In this article, we report on the development of a system for gene editing to create cows with a knockout of the beta-lactoglobulin gene.