1. Fluorescence anisotropy analysis of protein–antibody interaction
- Author
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Barbero, Nadia, Napione, Lucia, Quagliotto, Pierluigi, Pavan, Simona, Barolo, Claudia, Barni, Ermanno, Bussolino, Federico, and Viscardi, Guido
- Subjects
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FLUORIMETRY , *ANISOTROPY , *PROTEIN-protein interactions , *GLUTATHIONE transferase , *BIOCONJUGATES , *SPECTRUM analysis - Abstract
Abstract: The interaction between glutathione S-transferase and its antibody α-glutathione S-transferase (B-14) was studied using fluorescence anisotropy, subsequent to glutathione S-transferase bioconjugation with fluorescein-5-maleimide, leading to the determination of the dissociation and association binding constants, K d and K a; good binding specificity was observed between glutathione S-transferase and the antibody B-14. The use of spectroscopic techniques, fluorescence anisotropy in particular, is a useful and favourable tool to study biochemical problems. [Copyright &y& Elsevier]
- Published
- 2009
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