1. A near-infrared fluorescent probe for detection of exogenous and endogenous hydrogen peroxide in vivo.
- Author
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He, Yanqi, Miao, Lanxi, Yu, Le, Chen, Qiuling, Qiao, Yimu, Zhang, Jun-Feng, and Zhou, Ying
- Subjects
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FLUORESCENT probes , *HYDROGEN peroxide , *REACTIVE oxygen species , *OPTICAL properties , *TREATMENT programs , *IN vivo studies - Abstract
Using α-ketoamide moiety as a sensing unit, a near-infrared fluorescent probe (DCHP) for H 2 O 2 detection was developed. DCHP exhibited excellent in vitro selectivity for H 2 O 2 over other interference species, including reactive oxygen species, ions and biothiols, with remarkable colorimetric and fluorescence changes being observed. In vivo imaging tests showed that DCHP successfully detected exogenous and endogenous H 2 O 2 in living cells and C. elegans. Moreover, DCHP was utilized as an efficient NIR fluorescent tool to corroborate the accumulation of H 2 O 2 during inflammation in zebrafish, which was cause by copper-induced neuromast damage. The inflammation-sensitive activation along with the promising optical properties highlight the potential of DCHP in future therapeutic treatment programs. Image 1 • Using α-ketoamide moiety as a sensing unit, a near-infrared fluorescent H 2 O 2 probe was developed. • This probe exhibited excellent in vitro selectivity for H 2 O 2 over other interference species. • This probe successfully detected exogenous and endogenous H 2 O 2 in living cells and C. elegans. • This probe was utilized to corroborate the accumulation of H 2 O 2 during inflammation in zebrafish. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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