1. Cold exposure affects glucose metabolism, lipid droplet deposition and mitophagy in skeletal muscle of newborn goats.
- Author
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Su, Duo, Song, Yulong, Li, Die, Yang, Shizhong, Zhan, Siyuan, Zhong, Tao, Guo, Jiazhong, Cao, Jiaxue, Li, Li, Zhang, Hongping, and Wang, Linjie
- Subjects
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SKELETAL muscle , *GLUCOSE metabolism , *PHYSIOLOGICAL effects of cold temperatures , *GOATS , *NEWBORN infants , *MUSCLE metabolism , *INSULIN - Abstract
• Cold exposure increased energy metabolism, resulting in decreased glycogen content in skeletal muscle of newborn goats. • Transcriptome analysis found that cold exposure up-regulated the expression levels of genes related to glucose uptake, glycolysis, and fatty acid and TG synthesis, promoting lipid deposition in skeletal muscle. • Cold exposure induced mitophagy in skeletal muscle. Cold exposure is a common stressor for newborn goats. Skeletal muscle plays an important role in maintaining whole-body homeostasis of glucose and lipid metabolism. However, the molecular mechanisms underlying regulation of skeletal muscle of newborn goats by cold exposure remains unclear. In this study, we found a significant increase (P < 0.01) in serum glucagon levels after 24 h of cold exposure (COLD, 6°C), while glucose and insulin concentrations were significantly decreased (P < 0.01) compared to room temperature (RT, 25°C). Additionally, we found that cold exposure reduced glycogen content (P < 0.01) in skeletal muscle. Pathway enrichment analysis revealed that cold exposure activated skeletal muscle glucose metabolism pathways (including insulin resistance and the insulin signaling pathway) and mitophagy-related pathways. Cold exposure up-regulated the expression of genes involved in fatty acid and triglyceride synthesis, promoting skeletal muscle lipid deposition. Notably, cold exposure induced mitophagy in skeletal muscle. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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