1. Identification and expression pattern ofBmlark, a homolog of theDrosophilagenelarkinBombyx mori†
- Author
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Juan Li, Zi-Long Wang, Xingfu Zha, Jun Duan, Zhonghuai Xiang, Ping Zhao, and Qingyou Xia
- Subjects
DNA, Complementary ,Molecular Sequence Data ,Gene Expression ,Genes, Insect ,Biology ,Biochemistry ,Exon ,Endocrinology ,Genetics ,Animals ,Drosophila Proteins ,Tissue Distribution ,Amino Acid Sequence ,Cloning, Molecular ,Molecular Biology ,Gene ,Peptide sequence ,Electrophoresis, Agar Gel ,Expressed Sequence Tags ,Zinc finger ,Regulation of gene expression ,Expressed sequence tag ,Base Sequence ,Models, Genetic ,Sequence Homology, Amino Acid ,fungi ,Alternative splicing ,Intron ,RNA-Binding Proteins ,Exons ,Bombyx ,Introns ,Circadian Rhythm ,Alternative Splicing ,Gene Expression Regulation ,Insect Proteins ,Drosophila ,Carrier Proteins ,Software - Abstract
Two Bombyx mori isoforms of the gene lark, which is shown to play an important role in Drosophila circadian rhythms, were identified and named Bmlark-PA and Bmlark-PB, respectively. Bmlark-PA consists of 5 exons and encodes a protein of 343 amino acid residues which contains 3 functional domains: two RRM (RNA recognization motif) domains and an RTZF (retroviral-type zinc finger) and shares 72% identity with the Drosophila gene lark at the amino acid level. Bmlark-PB lacks the sequence between 118 and 791 nt of Bmlark-PA and codes for a protein of 68 amino acid residues, which contains no distinct functional domains. Alignments of the cDNAs of Bmlark to the genomic draft sequence of B. mori showed that the gene Bmlark had a single copy in the genome, suggesting that an alternative splicing mechanism occurs in the gene Bmlark. RT-PCR analysis indicated that Bmlark-PA was expressed only in late pupae and adult but Bmlark-PB was broadly expressed in many tissues and throughout the developmental stages from embryo to adult.
- Published
- 2005
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