1. Differential expression of four human dynamin-like protein variants in brain tumors
- Author
-
Shiuh-Lin Hwang, Chen-Hua Liao, Chang-Han Chen, Shen-Long Howng, Yi-Ren Hong, and Chen-Kung Chou
- Subjects
Adenoma ,Dynamins ,Male ,Molecular Sequence Data ,Coated vesicle ,Dynamin-Like Protein ,Biology ,Astrocytoma ,law.invention ,GTP Phosphohydrolases ,Mitochondrial Proteins ,law ,Genetics ,Meningeal Neoplasms ,Animals ,Humans ,splice ,Pituitary Neoplasms ,Amino Acid Sequence ,Differential expression ,Molecular Biology ,Polymerase chain reaction ,Sequence Homology, Amino Acid ,Brain Neoplasms ,Vesicle ,Genetic Variation ,Proteins ,Cell Biology ,General Medicine ,Molecular biology ,Reverse transcriptase ,Recombinant Proteins ,Aberrant protein ,Rats ,Gene Expression Regulation, Neoplastic ,Alternative Splicing ,Female ,Meningioma ,Microtubule-Associated Proteins ,Sequence Alignment - Abstract
Dynamin-like protein, a large GTP-binding protein, has recently been cloned, and studies have suggested that it is involved in the formation of coated vesicles. In this report, the differential expression of four human dynamin-like protein splice variants (HdynIV-wildtype [WT], -11, -26, and -37) from various brain tumors was identified by reverse transcription/polymerase chain reaction (RT-PCR). One novel variant (HdynIV-11), not described previously, was identified. The four alternatively spliced variants exhibited tissue specificity in normal tissues. The HdynIV-WT was strongly expressed in the brain, whereas HdynIV-37 was expressed in all tissues examined. Moreover, HdynIV-26 was dominant in the liver and apparently overexpressed in all astrocytomas and most meningiomas and adenomas. This report suggests that HdynIV-26 may cause aberrant protein trafficking and alter vesicle formation in brain tumors. Our results also suggest that dynamin-like protein is associated with various brain tumors and, more importantly, that aberrant expression of the HdynIV-26 variant may play a role in brain tumorigenesis.
- Published
- 2000