1. Infliximab exerts no direct hepatotoxic effect on HepG2 cells in vitro
- Author
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Henie M. J. Roelofs, Wilbert H.M. Peters, Hilbert S. de Vries, Rene H. M. te Morsche, Dirk J. de Jong, and Tineke de Heij
- Subjects
Hepatoblastoma ,Physiology ,Azathioprine ,Pharmacology ,Inflammatory bowel disease ,6-thioguanine ,6-mercaptopurine ,HepG2 cells ,media_common ,biology ,Mercaptopurine ,Liver Neoplasms ,Gastroenterology ,Antibodies, Monoclonal ,Hep G2 Cells ,Antibody ,Molecular gastro-enterology and hepatology Translational research [IGMD 2] ,medicine.drug ,Drug ,musculoskeletal diseases ,medicine.medical_specialty ,Cell Survival ,media_common.quotation_subject ,macromolecular substances ,In Vitro Techniques ,Molecular gastro-enterology and hepatology Pathogenesis and modulation of inflammation [IGMD 2] ,Sensitivity and Specificity ,Internal medicine ,medicine ,Humans ,Thioguanine ,Analysis of Variance ,Original Paper ,Dose-Response Relationship, Drug ,business.industry ,Hepatotoxicity ,Hepatology ,Inflammatory Bowel Diseases ,medicine.disease ,Infliximab ,digestive system diseases ,In vitro ,Methotrexate ,Immunology ,biology.protein ,business - Abstract
Contains fulltext : 108348.pdf (Publisher’s version ) (Open Access) BACKGROUND: Infliximab-induced hepatotoxicity is reported in several case studies involving patients with inflammatory bowel disease (IBD) and a direct hepatotoxic effect has been proposed. OBJECTIVE: The aim of this study was to determine the direct in vitro toxicity of infliximab. As a proof of principle the in vitro toxicity of thiopurines and methotrexate was also determined. METHODS: Cell survival curves and the half maximal inhibitory concentrations (IC(50)) were obtained after 24, 48 and 72 h of incubation in HepG2 cells with the IBD drugs azathioprine, 6-mercaptopurine, 6-thioguanine, methotrexate or infliximab by using the WST-1 cytotoxicity assay. RESULTS: No in vitro hepatotoxicity in HepG2 cells was seen with infliximab, while concentration-dependent cytotoxicity was observed when HepG2 cells were incubated with increasing concentrations of azathioprine, 6-mercaptopurine and 6-thioguanine. CONCLUSION: Infliximab alone or given in combination with azathioprine showed no direct hepatotoxic effect in vitro, indicating that the postulated direct hepatotoxicity of infliximab is unlikely. 01 juni 2012
- Published
- 2012
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