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1. Structurally related immunological effects of triterpenoid saponins

Author

M, Bernhardt, C, Sturm, K H, Shaker, D H, Paper, G, Franz, and K, Seifert

Subjects
Immunity, Cellular, Plants, Medicinal, Macrophages, Tetrazolium Salts, In Vitro Techniques, Saponins, Lymphocyte Activation, Triterpenes, Cell Line, Mice, Structure-Activity Relationship, Thiazoles, Phagocytosis, Mice, Inbred DBA, Animals, Female, Cell Division, Cells, Cultured, Spleen
Abstract

The influence of the triterpenoid saponins 1-10 has been investigated on murine spleenocytes in the lymphocyte transformation test and on murine macrophages in an phagocytosis assay. The lymphocyte transformation test and the phagocytosis assay showed that the tested compounds have no stimulating effect. However, a significant inhibition of lymphocyte proliferation by the triterpenoid saponins 2, 6 and 10 was demonstrated.

Published
2001

2. [Polysaccharides from Nerium oleander: structure and biological activity]

Author

B M, Müller, F, Rosskopf, D H, Paper, J, Kraus, and G, Franz

Subjects
Male, Mice, Inbred C3H, Magnetic Resonance Spectroscopy, Plants, Medicinal, Cell Survival, Plant Extracts, Hydrolysis, Macrophages, In Vitro Techniques, Antineoplastic Agents, Phytogenic, Methylation, Mice, Structure-Activity Relationship, Phagocytosis, Polysaccharides, Tumor Cells, Cultured, Animals, Immunologic Factors, Female, Protease Inhibitors, Lymphocytes, Mitogens, Limulus Test
Abstract

A water extraction of crushed leaves of Nerium oleander yielded 2.3% of a crude polysaccharide. The main fraction (67%) represents a pectic polysaccharide mainly composed of galacturonic acid besides rhamnose, arabinose and galactose. The polysaccharide structure was characterized by NMR, mild acid- and pectinase treatment combined with GC-MS analyses. In vivo tests for a possible antitumor activity did not result in a significant action. Investigation of immunomodulating activity brought some indications for mitogenic activity and a weak macrophage-mediated cytotoxicity. Increase of phagocytosis could not be doubtlessly assigned to the polysaccharide due to the high amount of endotoxin in the homogenous fraction OLE 2.

Published
1991