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Aim: Knowing the most frequent causative agents and their sensitivities to antibiotics may contribute to use of appropriate antibiotics. In this study, we aimed to investigate the agents and their antibiotic sensitivity which is isolated from intensive care unit of neurosurgery clinic. Materials and Methods: This retrospective study was performed from 2001 to 2005 at the Neurosurgery intensive care unit of Dicle University, Diyarbakir. The patients charts who were followed up more than 48 hours in intensive care unit and their culture results were investigated. Results: There was 457 of 2197 patients which were operated in neurosurgery clinic and it was obtained 1155 culture samples from these patients in five years period. 317 positive cultures were obtained from 123 patients including 73 cerebrospinal fluid, 45 wound, 47 deep tracheal aspirate, 96 were urine samples, 27 blood samples and 29 venous catheters samples. Mostly Staphilococcus aureus and coagulase negative staphylococcus (CNS) were growth in cultures. From the cerebrospinal fluidd, blood and wound the most growth bacteria was coagulase negative staphilococcus, from the urine Eschericha coli, from deep tracheal aspiration. Klebsiella pnömonia, and from venous catheters Pseudomonas aureginosa was mostly showed reproduction. Conclusion: S. aureus, CNS and P. aureginosa were mostly isolated bacteria in neurosurgery intensive care unit. Nosocomial intensive care unit infections can be decreased by appropriate preventive measures. [ABSTRACT FROM AUTHOR]