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Centric diatom taxa in the Cyclotella ocellata and C. comensis complexes show high morphological variability and often apparently continuous morphological transitions. In this study, we investigated natural assemblages of the C. ocellata/C. comensis complex from Hungarian and Croatian lakes and from Turkish streams using morphological and molecular methods. The studied assemblages contained cells with morphologies resembling C. ocellata as well as other, closely related, species: C. comensis, C. pseudocomensis, C. costei, and C. trichnoidea. The goal of our paper was to assess whether the observed morphological differences were due to intraspecific variability or suggest the existence of several, putatively distinct species.Ten morphometric characters were measured, which, either individually, or in pairs, did not differentiate the nominal taxa in our assemblages. However, multivariate discriminant analysis has revealed a group including C. ocellata and C. trichonidea morphologies could be separated from another containing C. comensis, C. pseudocomensis and C. costei.A nuclear (18S rDNA) and a chloroplast (rbcL) gene were amplified and partially sequenced from environmental DNA or from isolated cells. The sequences showed little variability among the assemblages and nominal species. Although general congruence of molecular and morphometric separation supports the species level separation of C. ocellata/trichonidea from the probably conspecific C. comensis/pseudocomensis/costei, sequence divergences between the groups are in the same range as within them, so that a conspecificity of all four taxa cannot be unequivocally excluded. © 2015 The International Society for Diatom Research.

A population of Amphora micrometra Giffen, collected in September 2002 from different habitats in Laguna Blanca in the Bolivian Altiplano, South America, has been examined using light transmission and scanning electron microscopy. The original description of this species is incomplete and insufficient to characterize the taxon in the light of the newly collected ultrastructural data. The holotype of A. micrometra was examined during the present study and compared to Bolivian specimens. An emended diagnosis of A. micrometra using all available information is provided. A taxonomic discussion based on available literature and the ecology of the taxon is also presented herein.

Two Cyclotella species were found during the electron microscopic investigation of core samples from Lake La Cruz. Cyclotella distinguenda Hustedt was subdominant in the layers 80-110 cms and C. delicatula Hustedt was frequent and abundant in the layers 20-180 cms. SEM studies revealed an interesting variation in their valve structure elements. Analyses of the morphological characteristics of these species on the basis of SEM micrographs (55 photos were taken from C. distinguenda and 95 from C. delicatula) was made it possible to complete their description. In this paper, the similarities and differences between Cyclotella distinguenda, C. delicatula and related taxa (Cyclotella azigzensis Flower, Gasse et Hakansson, C. costei Druart et Straub, C. cyclopuncta Hakansson et Carter, C. granulata Kulumbaeva et Genkal, C. plitvicensis Hustedt, C. wuethrichiana Druart et Straub) are discussed.