1. Performance of Whole Blood Stimulation Assays for the Quantification of SARS-CoV-2 Specific T-Cell Response: A Cross-Sectional Study.
- Author
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Bergami, Federica, Arena, Francesca, Pattonieri, Eleonora Francesca, Gregorini, Marilena, Meloni, Federica, Abelli, Massimo, Ticozzelli, Elena, Testa, Giorgia, Lilleri, Daniele, Cassaniti, Irene, and Baldanti, Fausto
- Subjects
SARS virus ,T cells ,SARS-CoV-2 ,COVID-19 - Abstract
Since the identification of the new severe acute respiratory syndrome virus 2 (SARS-CoV-2), a huge effort in terms of diagnostic strategies has been deployed. To date, serological assays represent a valuable tool for the identification of recovered COVID-19 patients and for the monitoring of immune response elicited by vaccination. However, the role of T-cell response should be better clarified and simple and easy to perform assays should be routinely introduced. The main aim of this study was to compare a home-made assay for whole blood stimulation with a standardized ELISpot assay design in our laboratory for the assessment of spike-specific T-cell response in vaccinated subjects. Even if a good correlation between the assays was reported, a higher percentage of responder subjects was reported for immunocompromised subjects with ELISpot assay (56%) than home-made whole blood stimulation assay (33%). Additionally, three commercial assays were compared with our home-made assay, reporting a good agreement in terms of both positive and negative results. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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