1. Utility of the Cobas® Plasma Separation Card as a Sample Collection Device for Serological and Virological Diagnosis of Hepatitis C Virus Infection
- Author
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Fernando Velásquez-Orozco, Paula Salmerón, Josep Quer, Joan Martínez-Campreciós, Adrián Najarro-Centeno, Francisco Rodriguez-Frias, Maria Buti, Àngels Esteban, Ariadna Rando-Segura, Tomas Pumarola-Sune, Institut Català de la Salut, [Velásquez-Orozco F, Salmeron P, Pumarola-Suñe T] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Rando-Segura A] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain. Servei d’Hepatologia. Servei de Microbiologia i Bioquímica, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Martínez-Camprecios J] Servei de Medicina Interna, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Najarro-Centeno A, Esteban À] Servei d’Hepatologia. Servei de Microbiologia i Bioquímica, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Unitat del Fetge, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. Servei de Bioquímica, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Quer J] Unitat del Fetge, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Buti M] Servei de Medicina Interna, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Unitat del Fetge, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. CIBER de Enfermedades Hepáticas y Digestivas (CIBEREHD), Instituto de Salud Carlos III, Madrid, Spain. [Rodríguez-Frías F] Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain. Servei d’Hepatologia. Servei de Microbiologia i Bioquímica, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Unitat del Fetge, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. Servei de Bioquímica, Vall d’Hebron Hospital Universitari, Barcelona, Spain. CIBER de Enfermedades Hepáticas y Digestivas (CIBEREHD), Instituto de Salud Carlos III, Madrid, Spain, and Vall d'Hebron Barcelona Hospital Campus
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Virus de l'hepatitis C - Diagnòstic ,0301 basic medicine ,Plasma separation card ,Hepatitis C virus ,030106 microbiology ,Clinical Biochemistry ,Otros calificadores::/diagnóstico [Otros calificadores] ,medicine.disease_cause ,Article ,virosis::virosis::infecciones por virus ARN::infecciones por Flaviviridae::hepatitis C [ENFERMEDADES] ,Serology ,03 medical and health sciences ,0302 clinical medicine ,Other subheadings::/diagnosis [Other subheadings] ,dried plasma spot ,medicine ,Otros calificadores::/instrumentación [Otros calificadores] ,lcsh:R5-920 ,medicine.diagnostic_test ,business.industry ,Sang - Anàlisi ,Antibody titer ,Virus Diseases::Virus Diseases::RNA Virus Infections::Flaviviridae Infections::Hepatitis C [DISEASES] ,Hepatitis C ,medicine.disease ,Virology ,Other subheadings::/instrumentation [Other subheadings] ,Titer ,diagnóstico::técnicas y procedimientos diagnósticos::técnicas de laboratorio clínico::pruebas de bioquímica clínica::análisis químico de la sangre::análisis de manchas de sangre seca [TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS] ,Immunoassay ,030211 gastroenterology & hepatology ,Sample collection ,Dried plasma spot ,hepatitis C ,lcsh:Medicine (General) ,business ,Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Clinical Chemistry Tests::Blood Chemical Analysis::Dried Blood Spot Testing [ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT] ,plasma separation card ,Viral load - Abstract
Taca de plasma sec; Hepatitis C; Targeta de separació de plasma Mancha de plasma seca; Hepatitis C; Tarjeta de separación de plasma Dried plasma spot; Hepatitis C; Plasma separation card Diagnosis and clinical management of people infected with hepatitis C virus (HCV) relies on results from a combination of serological and virological tests. The aim of this study was to compare the performance of dried plasma spots (DPS), prepared using the cobas® Plasma Separation Card (PSC), to plasma and serum from venipuncture, for HCV diagnosis. We carried out a prospective study using DPS and paired plasma or serum samples. Serum and DPS samples were analyzed by immunoassay using Elecsys® Anti-HCV II (Roche). Plasma and DPS samples were analyzed using the cobas® HCV viral load and cobas® HCV genotyping tests (Roche). All DPS samples that had high anti-HCV antibody titers in serum were also antibody-positive, as were five of eight samples with moderate titers. Eight samples with low titers in serum were negative with DPS. Among 80 samples with plasma HCV viral loads between 61.5 and 2.2 × 108 IU/mL, 74 were RNA-positive in DPS. The mean viral load difference between plasma and DPS was 2.65 log10 IU/mL. The performance of DPS for detection of serological and virological markers of hepatitis C virus infection was comparable to that of the conventional specimen types. However, the limits of detection were higher for DPS. This work was supported by Roche and a microelimiation grant of Gilead Science (GLD19-0104).
- Published
- 2021
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