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Start Over Author "Xingfu Li" Journal diagnostic pathology
4 results on '"Xingfu Li"'

1. Increased Tim-3 expression on peripheral T lymphocyte subsets and association with higher disease activity in systemic lupus erythematosus

Author

Qiang Shu, Lijun Song, Dong Li, Xingfu Li, Xiao Wang, Xiao Yu, Xu-ping Wang, Huaxiang Liu, and Feng Ding

Subjects
Male, Pathology, Severity of Illness Index, Fas ligand, Pathogenesis, T-Lymphocyte Subsets, Lupus Erythematosus, Systemic, Disease activity, skin and connective tissue diseases, Hepatitis A Virus Cellular Receptor 2, biology, Reverse Transcriptase Polymerase Chain Reaction, Complement C4, General Medicine, Complement C3, Middle Aged, Flow Cytometry, Prognosis, Up-Regulation, medicine.anatomical_structure, Female, Adult, medicine.medical_specialty, Histology, Fas Ligand Protein, CD3, T cell, Galectins, Tim-3, Real-Time Polymerase Chain Reaction, Peripheral blood mononuclear cell, Pathology and Forensic Medicine, Young Adult, Systemic lupus erythematosus, T lymphocyte subset, medicine, Humans, RNA, Messenger, fas Receptor, Galectin, Lupus erythematosus, Research, Membrane Proteins, Fas, medicine.disease, Case-Control Studies, Immunology, biology.protein, Biomarkers, Anti-SSA/Ro autoantibodies
Abstract

Background Both the T cell immunoglobulin domain- and mucin domain-containing molecule-3 (Tim-3) and the death receptor Fas contribute to the pathogenesis of various autoimmune diseases, including systemic lupus erythematosus (SLE). The aim of the present study was to determine whether Tim-3 and Fas are co-expressed on certain peripheral T lymphocyte subsets, and whether this expression is associated with greater disease activity in SLE. Methods Peripheral blood mononuclear cells were isolated from 46 patients newly diagnosed with SLE and 28 age- and sex-matched healthy controls (HCs). Expression of Tim-3 and Fas on T subsets was analyzed by flow cytometry, while mRNA levels of the Tim-3 ligand galectin-9 and Fas ligand FasL were assayed using real-time RT-PCR. Results The proportions of CD3+CD4+ and CD3+CD4- T cells expressing Tim-3+ and Tim+Fas+ were significantly higher in patients than in HCs (p

Published
2014

2. The positive correlations of apolipoprotein E with disease activity and related cytokines in systemic lupus erythematosus

Author

Lijun Song, Qilin Chen, Feng Qiu, Xingfu Li, Feng Ding, Weiwei Liu, and Yu-Chen Fan

Subjects
Adult, Male, Apolipoprotein E, medicine.medical_specialty, Histology, Down-Regulation, Severity of Illness Index, Peripheral blood mononuclear cell, Pathology and Forensic Medicine, chemistry.chemical_compound, Systemic lupus erythematosus, Apolipoproteins E, Prednisone, Internal medicine, medicine, Humans, Lupus Erythematosus, Systemic, Anti-inflammatory cytokine, skin and connective tissue diseases, Glucocorticoids, SLEDAI, Lupus erythematosus, Triglyceride, biology, business.industry, Research, Case-control study, DNA, General Medicine, Middle Aged, medicine.disease, Antibodies, Anti-Idiotypic, Treatment Outcome, Endocrinology, chemistry, Case-Control Studies, Immunology, biology.protein, Cytokines, Female, lipids (amino acids, peptides, and proteins), Antibody, business, Biomarkers, Glucocorticoid, medicine.drug
Abstract

Background The aim of this study is to investigate the expression of apolipoprotein E (apoE) and the relationship between apoE and disease activity of SLE, and the possible effects of glucocorticoid on apoE and other cytokines activities in SLE patients. Methods Forty treatment-naïve SLE patients and forty matched healthy controls were studied. All the SLE patients received prednisone 1 mg/kg/day for 28 consecutive days. The sera levels of apoE and related cytokines were evaluated by ELISA. The expression of apoE mRNA in peripheral blood mononuclear cells (PBMCs) was determined by real-time PCR. Results Compared with healthy controls, the relative expression levels of ApoE proteins and sera levels were significantly up-regulated in active SLE patients. ApoE sera concentrations positively correlated with SLEDAI, anti-dsDNA antibody and the related cytokines including IL-6, IFN-γ and IL-10, and uncorrelated with the concentration of total cholesterol (TC) and triglyceride (TG) in SLE patients. After 4 weeks prednisone treatment, the relative mRNA expression of apoE and the serum levels of apoE and related cytokines decreased. Conclusions ApoE correlated with disease activity and related cytokines in SLE patients. Glucocorticoid can down-regulate the expressions of apoE and related cytokines. Virtual slide The virtual slide(s) for this article can be found here:http://www.diagnosticpathology.diagnomx.eu/vs/1646714011077325

Published
2013
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3. Expression level of the growth factor progranulin is related with development of systemic lupus erythematosus.

Author

Feng Qiu, Lijun Song, Feng Ding, Huaxiang Liu, Qiang Shu, Ning Yang, Weiwei Liu, and Xingfu Li

Subjects
GROWTH factors, PROGRANULIN, SYSTEMIC lupus erythematosus, MESSENGER RNA, CYTOKINES
Abstract

Background: This study is to investigate the expression of progranulin (PGRN) in systemic lupus erythematosus (SLE) patients and the effect of glucocorticoid (GC) treatment on its expression. Methods: Thirty newly diagnosed severe SLE patients and 30 healthy subjects were enrolled in this study. The serum levels of PGRN and the inflammatory factors of SLE were detected by ELISA and the mRNA expression of these proteins were detected by real-time PCR. Results: The serum levels of PGRN, IL-6, PR3, TNFR, TNF-α and anti-dsDNA antibody in SLE patients were increased significantly compared with healthy controls (P < 0.05). The relative expression of PGRN mRNA was increased by 4.88-fold in pre-treatment SLE patients compared with controls (P < 0.05). After prednisone treatment, the serum levels of PGRN decreased significantly, and the relative expression of PGRN mRNA was decreased by 1.34-fold compared with the untreated controls (P < 0.01). Moreover, Serum concentration of PGRN was correlated with serum levels of IL-6, TNF-α, TNFR and anti-dsDNA antibody in both pre-treatment and post-treatment SLE patients. Conclusions: PGRN is up-regulated in the SLE patients and is correlated with pro-inflammatory cytokines and anti-dsDNA antibody. Glucocorticoids can down-regulate the expression of PGRN in SLE patients. [ABSTRACT FROM AUTHOR]

Published
2013
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4. Expression level of the growth factor progranulin is related with development of systemic lupus erythematosus

Author

Ning Yang, Huaxiang Liu, Feng Qiu, Xingfu Li, Weiwei Liu, Feng Ding, Qiang Shu, and Lijun Song

Subjects
Adult, Male, Progranulin, Histology, Adolescent, medicine.medical_treatment, Inflammation, Pathology and Forensic Medicine, Young Adult, Progranulins, Systemic lupus erythematosus, Glucocorticoid, Downregulation and upregulation, Prednisone, immune system diseases, medicine, Humans, Lupus Erythematosus, Systemic, Interleukin 6, skin and connective tissue diseases, IL-6, Lupus erythematosus, biology, business.industry, Interleukin-6, Tumor Necrosis Factor-alpha, Growth factor, Research, General Medicine, Middle Aged, medicine.disease, Up-Regulation, Immunology, biology.protein, Intercellular Signaling Peptides and Proteins, Tumor necrosis factor alpha, Female, medicine.symptom, business, medicine.drug
Abstract

Background This study is to investigate the expression of progranulin (PGRN) in systemic lupus erythematosus (SLE) patients and the effect of glucocorticoid (GC) treatment on its expression. Methods Thirty newly diagnosed severe SLE patients and 30 healthy subjects were enrolled in this study. The serum levels of PGRN and the inflammatory factors of SLE were detected by ELISA and the mRNA expression of these proteins were detected by real-time PCR. Results The serum levels of PGRN, IL-6, PR3, TNFR, TNF-α and anti-dsDNA antibody in SLE patients were increased significantly compared with healthy controls (P Conclusions PGRN is up-regulated in the SLE patients and is correlated with pro-inflammatory cytokines and anti-dsDNA antibody. Glucocorticoids can down-regulate the expression of PGRN in SLE patients. Virtual slides http://www.diagnosticpathology.diagnomx.eu/vs/1562484036905973

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