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Your search keyword '"Plasmodium malariae classification"' showing total 2 results
Start Over Descriptor "Plasmodium malariae classification" Journal diagnostic microbiology and infectious disease
2 results on '"Plasmodium malariae classification"'

1. Malaria surveillance in the Democratic Republic of the Congo: comparison of microscopy, PCR, and rapid diagnostic test.

Author

Doctor SM, Liu Y, Whitesell A, Thwai KL, Taylor SM, Janko M, Emch M, Kashamuka M, Muwonga J, Tshefu A, and Meshnick SR

Subjects
Democratic Republic of the Congo epidemiology, Diagnostic Tests, Routine methods, Diagnostic Tests, Routine standards, Humans, Malaria epidemiology, Microscopy, Plasmodium malariae cytology, Plasmodium malariae genetics, Polymerase Chain Reaction, Public Health Surveillance, Sensitivity and Specificity, Malaria diagnosis, Malaria parasitology, Plasmodium malariae classification
Abstract

Malaria surveillance is critical for control efforts, but diagnostic methods frequently disagree. Here, we compare microscopy, PCR, and a rapid diagnostic test in 7137 samples from children in the Democratic Republic of the Congo using latent class analysis. PCR had the highest sensitivity (94.6%) and microscopy had the lowest (76.7%)., (Copyright © 2016 Elsevier Inc. All rights reserved.)

Published
2016
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2. The potential utility of the Semi-Nested Multiplex PCR technique for the diagnosis and investigation of congenital malaria.

Author

Rubio JM, Roche J, Berzosa PJ, Moyano E, and Benito A

Subjects
Adult, Animals, Diseases in Twins, Female, Humans, Infant, Newborn, Malaria parasitology, Malaria, Falciparum congenital, Malaria, Falciparum transmission, Male, Plasmodium genetics, Plasmodium isolation & purification, Plasmodium falciparum classification, Plasmodium falciparum genetics, Plasmodium falciparum isolation & purification, Plasmodium malariae classification, Plasmodium malariae genetics, Plasmodium malariae isolation & purification, Pregnancy, Infectious Disease Transmission, Vertical, Malaria congenital, Malaria transmission, Plasmodium classification, Pregnancy Complications, Parasitic
Abstract

We report three cases of congenital malaria involving two malarial immune mothers living in Spain. Diagnostic PCR and Genotyping PCR for merozoite surface proteins 1 and 2 were essential to show that mothers and new-borns had different Plasmodium population parasites at the moment of the delivery, and that the infection was acquired earlier in gestation by transplacental transmission. In the first case the Plasmodium species founded in both, mother and child were different. Malaria in the twins showed a mixed infection (P. falciparum plus P. malariae) while the mother presented a P. falciparum infection. These facts were confirmed studying the polymorphisms for MSP1 and MSP2. Blood samples of the newborns were analyzed an half hour after delivery excluding the possibility of re-infection by mosquito bite and indicating a vertical transmission during pregnancy.

Published
2000
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