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Your search keyword '"Magdalena A. Taracila"' showing total 2 results
Start Over Author "Magdalena A. Taracila" Journal diagnostic microbiology and infectious disease
2 results on '"Magdalena A. Taracila"'

1. A comprehensive and contemporary 'snapshot' of β-lactamases in carbapenem resistant Acinetobacter baumannii

Author

Thomas H. Clarke, Derrick E. Fouts, Magdalena A. Taracila, Pratap Venepally, David A. Leonard, Bradley J. Wallar, Paul G. Higgins, Rachel A. Powers, Philip N. Rather, Lauren Brinkac, Robert A. Bonomo, Steven H. Marshall, Andrew R Mack, Kristine M. Hujer, Barry N. Kreiswirth, Fabio Prati, Christopher Greco, Emilia Caselli, and Andrea M. Hujer

Subjects
Acinetobacter baumannii, 0301 basic medicine, Microbiology (medical), 030106 microbiology, OXA carbapenemase, beta-Lactam Resistance, beta-Lactamases, Microbiology, carbapenemase, 03 medical and health sciences, 0302 clinical medicine, Bacterial Proteins, Carbapenem resistant Acinetobacter baumannii, Humans, 030212 general & internal medicine, Allele, OXA β-lactamase, OXA-23, biology, OXA-82, β lactamases, ADC β-lactamase, OXA-172, General Medicine, Antimicrobial, biology.organism_classification, Infectious Diseases, Carbapenems, Original Article, carbapenem resistant Acinetobacter baumannii, Genome, Bacterial, Acinetobacter Infections
Abstract

Successful treatment of Acinetobacter baumannii infections require early and appropriate antimicrobial therapy. One of the first steps in this process is understanding which β-lactamase (bla) alleles are present and in what combinations. Thus, we performed WGS on 98 carbapenem-resistant A. baumannii (CR Ab). In most isolates, an acquired blaOXA carbapenemase was found in addition to the intrinsic blaOXA allele. The most commonly found allele was blaOXA-23 (n = 78/98). In some isolates, blaOXA-23 was found in addition to other carbapenemase alleles: blaOXA-82 (n = 12/78), blaOXA-72 (n = 2/78) and blaOXA-24/40 (n = 1/78). Surprisingly, 20% of isolates carried carbapenemases not routinely assayed for by rapid molecular diagnostic platforms, i.e., blaOXA-82 and blaOXA-172; all had ISAba1 elements. In 8 CR Ab, blaOXA-82 or blaOXA-172 was the only carbapenemase. Both blaOXA-24/40 and its variant blaOXA-72 were each found in 6/98 isolates. The most prevalent ADC variants were blaADC-30 (21%), blaADC-162 (21%), and blaADC-212 (26%). Complete combinations are reported.

Published
2021

2. Predicting β-lactam resistance using whole genome sequencing in Klebsiella pneumoniae: the challenge of β-lactamase inhibitors

Author

Randall J. Olsen, James M. Musser, Andrea M. Hujer, S. Wesley Long, Laura J. Rojas, Magdalena A. Taracila, and Robert A. Bonomo

Subjects
0301 basic medicine, Microbiology (medical), Genotype, Klebsiella pneumoniae, Avibactam, 030106 microbiology, Aztreonam, Biology, beta-Lactams, Gene Expression Regulation, Enzymologic, beta-Lactamases, Article, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Antibiotic resistance, Drug Resistance, Multiple, Bacterial, Amino Acid Sequence, 030212 general & internal medicine, Beta-Lactamase Inhibitors, Whole Genome Sequencing, Gene Expression Regulation, Bacterial, General Medicine, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, biology.organism_classification, Phenotype, Anti-Bacterial Agents, Infectious Diseases, chemistry, Ceftolozane, beta-Lactamase Inhibitors, Genome, Bacterial
Abstract

Although multiple antimicrobial resistance (AMR) determinants can confer the same in vitro antimicrobial susceptibility testing (AST) phenotype, their differing effect on optimal therapeutic choices is uncertain. Using a large population-based collection of clinical strains spanning a 3.5-year period, we applied WGS to detect inhibitor resistant (IR), extended-spectrum β-lactamase (ESBL), and carbapenem resistant (CR) β-lactamase (bla) genes and compared the genotype to the AST phenotype in select isolates. All blaNDM-1 (9/9) and the majority of blaNDM-1/OXA-48 (3/4) containing isolates were resistant to CAZ/AVI as predicted by WGS. The combination of ATM and CAZ/AVI restored susceptibility by disk diffusion assay. Unexpectedly, clinical Kp isolates bearing blaKPC-8 (V240G) and blaKPC-14 (G242 and T243 deletion) did not test fully resistant to CAZ/AVI. Lastly, despite the complexity of the β-lactamase background, CAZ/AVI retained potency. Presumed phenotypes conferred by AMR determinants need to be tested if therapeutic decisions are being guided by their presence or absence.

Published
2020

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