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Your search keyword '"Belhadj, Omrane"' showing total 3 results
Start Over Author "Belhadj, Omrane" Journal diagnostic microbiology and infectious disease
3 results on '"Belhadj, Omrane"'

3. Cloning and sequencing of the class A beta-lactamase gene (blaTEM-15) located on a chromosomal Tn801 transposon.

Author

Chouchani C, Ben-Achour N, M'Charek A, and Belhadj O

Subjects
Base Sequence, Cloning, Molecular, DNA Transposable Elements, Escherichia coli isolation & purification, Escherichia coli Proteins chemistry, Escherichia coli Proteins genetics, Feces microbiology, Humans, Infant, Isoelectric Point, Microbial Sensitivity Tests, Molecular Sequence Data, Sequence Analysis, DNA, beta-Lactamases chemistry, beta-Lactams pharmacology, Escherichia coli drug effects, Escherichia coli genetics, Escherichia coli Infections microbiology, beta-Lactam Resistance genetics, beta-Lactamases genetics
Abstract

Escherichia coli CA0210 was identified in a stool culture of a 03-month-neonate in Tunisia. This strain was resistant to beta-lactams, including ureidopenicillins, ticarcillin-clavulanic acid, cefpirome, ceftazidime, and cefotaxime, but it remained susceptible to imipenem and cefoxitin. The beta-lactam-hydrolyzing beta-lactamase gene of E. coli CA0210 and the upstream and downstream regions were cloned, sequenced, and expressed in E. coli DH5alpha. These resistances were carried by a 1080-bp chromosomal gene that encoded a beta-lactamase with a pI of 6.3. Cloning and sequencing experiments showed that the corresponding blaTEM-15 gene was part of a chromosomally located Tn801 transposon.

Published
2007
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