1. Prevalence and characterization of hybrid blaCTX-M among Escherichia coli isolates from livestock and other animals.
- Author
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Ho, Pak-Leung, Liu, Melissa Chun-Jiao, Lo, Wai-U, Lai, Eileen Ling-Yi, Lau, Terrence Chi-Kong, Law, Oi-Kwan, and Chow, Kin-Hung
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DISEASE prevalence , *ESCHERICHIA coli physiology , *LIVESTOCK , *BETA lactamases , *PLASMIDS , *RESTRICTION fragment length polymorphisms - Abstract
This study investigated 248 extended-spectrum β-lactamase-producing Escherichia coli isolates from 2012 to 2013 for hybrid bla CTX-M genes. bla CTX-M genes were detected in 228 isolates of which 14 isolates were hybrid bla CTX-M positive (6 bla CTX-M-123 , 6 bla CTX-M-64 , and 2 bla CTX-M-132 ). The 14 hybrid bla CTX-M –carrying isolates (8 from chickens, 2 each from pigs and cattle, 1 each from dog and rodent) were genetically diverse. All but 2 hybrid bla CTX-M were carried on IncI1 (5 bla CTX-M-123 ) and IncI2 (6 bla CTX-M-64 and one bla CTX-M-132 ) plasmids. Our IncI1 and IncI2 plasmids had pHNAH4-1–like and pHN1122-1–like restriction fragment length polymorphism patterns, respectively. Genetic relatedness of the plasmids to pHNAH4-1 and pHN1122-1 were confirmed by complete sequencing of 3 plasmids, pCTXM123_C0996, pCTXM64_C0967, and pCTXM132_P0421. Plasmids closely related to pHNAH4-1 and pHN1122-1 and carrying different bla CTX-M alleles have been reported from multiple geographic areas in China previously. The findings highlighted the wide dissemination of hybrid bla CTX-M variants in different parts of China. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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