1. Mutations in the beta1 adrenergic receptor gene and massive obesity in Japanese
- Author
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Kishio Nanjo, Nobuyuki Takasu, Hiroto Furuta, Koichi Yabiku, Yuzuru Ohshiro, Masahiro Nishi, Hisao Wakasaki, Hideyuki Sasaki, Mina Hayashi, Masayuki Ishigame, and Kazuya Ueda
- Subjects
medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,medicine.disease_cause ,Polymerase Chain Reaction ,Polymorphism, Single Nucleotide ,Beta-1 adrenergic receptor ,Endocrinology ,Asian People ,Japan ,Polymorphism (computer science) ,Internal medicine ,Genetic variation ,Internal Medicine ,Medicine ,Animals ,Humans ,Obesity ,DNA Primers ,Mutation ,business.industry ,Homozygote ,Genetic Variation ,General Medicine ,medicine.disease ,Obesity, Morbid ,Rats ,Amino Acid Substitution ,Models, Animal ,Restriction fragment length polymorphism ,Receptors, Adrenergic, beta-1 ,business ,Thermogenesis ,Body mass index ,Polymorphism, Restriction Fragment Length - Abstract
Catecholamines strongly promote lipolysis and thermogenesis, and play a central role in the regulation of body fat content. The beta1 adrenergic receptor (BAR-1) is a major mediator of catecholamine-induced lipolysis and thermogenesis. To explore whether mutations in the BAR-1 gene contribute to morbid obesity in Japanese, we scanned for mutations in the coding sequence of the gene in 50 morbid obese [body mass index (BMI)>==35.0kg/m(2); 99.7th percentile] Japanese subjects. Direct DNA sequencing was performed following polymerase chain reaction (PCR) amplification. Two common polymorphisms, Gly49Arg and Arg389Ser, were detected in these subjects. The frequencies of these polymorphisms, as determined by PCR-restriction fragment length polymorphism (RFLP) analysis, showed no significant difference between 180 severely obese subjects (BMI>==30.0kg/m(2); 97th percentile) and 132 control (BMI
- Published
- 2007