1. Evaluation of the brain and kidney renin-angiotensin system and oxidative stress in neonatal handled rats.
- Author
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Rodriguez DL, de Mesquita FC, Attolini D, de Borba BS, Scherer PS, Almeida PH, da Costa VL, Scherer BS, Schmitt VM, de Oliveira JR, and Donadio MV
- Subjects
- Angiotensin II metabolism, Animals, Animals, Newborn, Catalase metabolism, Female, Male, Malondialdehyde metabolism, Rats, Rats, Wistar, Receptors, Angiotensin metabolism, Renin metabolism, Reverse Transcriptase Polymerase Chain Reaction, Superoxide Dismutase metabolism, Brain metabolism, Handling, Psychological, Kidney metabolism, Oxidative Stress physiology, RNA, Messenger analysis, Renin-Angiotensin System physiology
- Abstract
The aim of this study was to test the hypothesis that the renin-angiotensin system (RAS) components, as well as the oxidative stress system, would respond to early environmental changes. Thus, we have evaluated the effects of neonatal handling on both brain and kidney RAS and oxidative stress. Pups were divided into two groups: nonhandled and handled. The procedure consisted of handling them for 1 min/day in the first 10 days of life. On days 1, 5, and 10, animals were killed by decapitation. Blood samples were collected and the brain and kidneys were removed. Renin, AT(1), and AT(2) mRNA expression were evaluated through RT-PCR. Angiotensin II (ANG II) serum concentration was also measured. An increased ANG II concentration, brain and kidney AT(2) mRNA expression were demonstrated. The kidney mRNA AT(1) expression was decreased. There was also a kidney lipid peroxidation increase and a brain superoxide dismutase and catalase decrease. In conclusion, handling in the neonatal period induces the activation of the angiotensinergic system, as well as modulates its mRNA receptor expression. The oxidative stress balance system seems not to be involved., (Copyright © 2011 Wiley Periodicals, Inc.)
- Published
- 2012
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