1. Migratory behavior of presumptive interneurons is affected by AMPA receptor activation in slice cultures of embryonic mouse neocortex
- Author
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Masato Yozu, Kazunori Nakajima, Hidenori Tabata, Norbert König, Gifu Academy of Forest Science and Culture, Laboratoire de neurogénétique, Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR122, School of Applied and Engineering physics [Ithaca] (AEP Cornell), Cornell University [New York], and Dept. of Electrical and Computer Engineering
- Subjects
MESH: Dose-Response Relationship, Drug ,Mice ,0302 clinical medicine ,Cell Movement ,MESH: Gene Expression Regulation, Developmental ,Excitatory Amino Acid Agonists ,MESH: Animals ,Receptor ,MESH: Cell Movement ,[SDV.BDD]Life Sciences [q-bio]/Development Biology ,Cerebral Cortex ,0303 health sciences ,Mice, Inbred ICR ,Neocortex ,MESH: alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid ,musculoskeletal, neural, and ocular physiology ,Stem Cells ,Glutamate receptor ,Gene Expression Regulation, Developmental ,Cell Differentiation ,Transfection ,MESH: Interneurons ,medicine.anatomical_structure ,Neurology ,Cerebral cortex ,MESH: Receptors, AMPA ,Female ,MESH: Cell Differentiation ,Ganglionic eminence ,AMPA receptor ,MESH: Stem Cells ,Biology ,03 medical and health sciences ,Organ Culture Techniques ,Developmental Neuroscience ,Interneurons ,medicine ,Animals ,Cell Lineage ,Receptors, AMPA ,MESH: Mice, Inbred ICR ,MESH: Mice ,alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid ,030304 developmental biology ,Dose-Response Relationship, Drug ,MESH: Transfection ,MESH: Cell Lineage ,Embryonic stem cell ,MESH: Organ Culture Techniques ,MESH: Cerebral Cortex ,nervous system ,MESH: Excitatory Amino Acid Agonists ,Neuroscience ,MESH: Female ,030217 neurology & neurosurgery - Abstract
It has been reported that functional α-amino-3-hydroxy-5-methyl-isoxazolpro-prionic acid (AMPA) receptors permeable to calcium are already expressed by tangentially migrating prospective interneurons in the developing rodent cerebral cortex. However, the role of these receptors is not yet fully understood. To examine the effect of activation of AMPA receptors on tangential migration, we exposed migrating prospective interneurons derived from the medial ganglionic eminence (MGE) to AMPA in slice cultures and performed time lapse imaging. In the neocortex, a subpopulation of MGE-derived cells stopped migration or changed the direction of migration in response to AMPA in a dose-dependent manner. In contrast, neither MGE-derived cells migrating in the subcortical territory nor radially migrating cells in the neocortex were affected by exposure to AMPA. Transfection of dominant-negative AMPA receptor subunit GluR1 to the tangentially migrating cells prevented the effects of AMPA on migration. This study provides evidence that the activation of AMPA receptors can directly affect tangential migration in the neocortical part of telencephalic slice cultures.
- Published
- 2006