1. Effect of lncRNA H19 on nerve degeneration and regeneration after sciatic nerve injury in rats
- Author
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Yi Wang, Nannan Gao, Xi Xu, Huanhuan Zhang, Min Cai, Bryant Yung, Yumei Feng, Dengbing Yao, Yuting Li, and Huiwei Huang
- Subjects
Wallerian degeneration ,Biology ,Rats, Sprague-Dawley ,Cellular and Molecular Neuroscience ,Developmental Neuroscience ,Dorsal root ganglion ,Peripheral Nerve Injuries ,medicine ,Animals ,Regeneration (biology) ,Wnt signaling pathway ,Sciatic nerve injury ,medicine.disease ,Sciatic Nerve ,Cell biology ,Nerve Regeneration ,Rats ,medicine.anatomical_structure ,nervous system ,Apoptosis ,embryonic structures ,Nerve Degeneration ,RNA, Long Noncoding ,Sciatic nerve ,Schwann Cells ,Signal transduction ,Wallerian Degeneration - Abstract
Hundreds of millions of people worldwide suffer from peripheral nerve damage resulting from car accidents, falls, industrial accidents, residential accidents, and wars. The purpose of our study was to further investigate the effects of Wallerian degeneration (WD) after rat sciatic nerve injury and to screen for critical long noncoding RNAs (lncRNAs) in WD. We found H19 to be essential for nerve degeneration and regeneration and to be highly expressed in the sciatic nerves of rats with WD. LncRNA H19 potentially impaired the recovery of sciatic nerve function in rats. H19 was mainly localized in the cytoplasm of Schwann cells (SCs) and promoted their migration. H19 promoted the apoptosis of dorsal root ganglion (DRG) neurons and slowed the growth of DRG axons. The lncRNA H19 may play a role in WD through the Wnt/β-catenin signaling pathway and is coexpressed with a variety of crucial mRNAs during WD. These data provide further insight into the molecular mechanisms of WD. This article is protected by copyright. All rights reserved.
- Published
- 2021