1. Regulation of plant stem cell quiescence by a brassinosteroid signaling module
- Author
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Norma Fàbregas, Jan U. Lohmann, Philip N. Benfey, Marta Ibañes, Josep Vilarrasa-Blasi, Konstantinos G. Alexiou, David Frigola, Alain Jauneau, Susana Rivas, Mary Paz González-García, Ana I. Caño-Delgado, Nuria Lopez-Bigas, Generalitat de Catalunya, German Research Foundation, Ministerio de Economía y Competitividad (España), Agence Nationale de la Recherche (France), Ministerio de Ciencia e Innovación (España), National Science Foundation (US), European Commission, Centre de Recerca en Agrigenòmica - Center for Research in Agricultural Genomics, Partenaires INRAE, University of Barcelona, Barcelona Biomedical Research Park (PRBB), Unité mixte de recherche interactions plantes-microorganismes, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Fédération de Recherche Agrobiosciences, Interactions et Biodiversité (FR AIB), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Heidelberg University, Duke University [Durham], FI PhD fellowship from the Generalitat de Catalunya (GC), DFG [BE1-00924, SFB873], Spanish Ministry de Economy and Competitiveness [FIS2012-37655-C02-02], GC [2009SGR14], Laboratoire d'Excellence (LABEX) TULIP [ANR-10-LABX-41], 'Juan de la Cierva' post-doctoral contract from the Spanish Ministry of Science HFSP short-term fellowship, NSF Arabidopsis grant, Spanish Ministry of Innovation and Science [BIO2010/007], and Marie-Curie Initial Training Network 'BRAVISSIMO' [PITN-GA-2008-215118, FPU-AP2009-3736]
- Subjects
Cell division ,[SDV]Life Sciences [q-bio] ,Arabidopsis ,Plant Roots ,chemistry.chemical_compound ,0302 clinical medicine ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,Brassinosteroid ,Stem Cell Niche ,Oligonucleotide Array Sequence Analysis ,2. Zero hunger ,0303 health sciences ,Reverse Transcriptase Polymerase Chain Reaction ,Stem Cells ,Plants, Genetically Modified ,Cell biology ,Signal transduction ,Stem cell ,Cell Division ,Signal Transduction ,Plant stem cell ,Chromatin Immunoprecipitation ,animal structures ,Blotting, Western ,Enzyme-Linked Immunosorbent Assay ,Biology ,Real-Time Polymerase Chain Reaction ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Brassinosteroids ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Immunoprecipitation ,RNA, Messenger ,Molecular Biology ,Transcription factor ,030304 developmental biology ,Cell Proliferation ,Cell growth ,Arabidopsis Proteins ,Gene Expression Profiling ,Cell Biology ,Models, Theoretical ,biology.organism_classification ,chemistry ,Mutation ,030217 neurology & neurosurgery ,Biomarkers ,Developmental Biology - Abstract
Referred to by: Josep Vilarrasa-Blasi, Mary-Paz González-García, David Frigola, Norma Fàbregas-Vallvé, Konstantinos G. Alexiou, Nuria López-Bigas, Susana Rivas, Alain Jauneau, Jan U. Lohmann, Philip N. Benfey, Marta Ibañes, Ana I. Caño-Delgado Regulation of Plant Stem Cell Quiescence by a Brassinosteroid Signaling Module Developmental Cell, Volume 33, Issue 2, 20 April 2015, Pages 238., The quiescent center (QC) maintains the activity of the surrounding stem cells within the root stem cell niche, yet specific molecular players sustaining the low rate of QC cell division remain poorly understood. Here, we identified a R2R3-MYB transcription factor, BRAVO (BRASSINOSTEROIDS AT VASCULAR AND ORGANIZING CENTER), acting as a cell-specific repressor of QC divisions in the primary root of Arabidopsis. Ectopic BRAVO expression restricts overall root growth and ceases root regeneration upon damage of the stem cells, demonstrating the role of BRAVO in counteracting Brassinosteroid (BR)-mediated cell division in the QC cells. Interestingly, BR-regulated transcription factor BES1 (BRI1-EMS SUPRESSOR 1) directly represses and physically interacts with BRAVO in vivo, creating a switch that modulates QC divisions at the root stem cell niche. Together, our results define a mechanism for BR-mediated regulation of stem cell quiescence in plants., J.V.-B. and N.F.-V. are funded by FI PhD fellowship from the Generalitat de Catalunya (GC) in the A.I.C.-D. laboratory. J.V.-B. received a short-term fellowship (BE1-00924) in the Lohmann (J.U.L.) laboratory supported by the SFB873 of the DFG. Research by D.F and M.I. is funded by FIS2012-37655-C02-02 by the Spanish Ministry de Economy and Competitiveness and 2009SGR14 from GC, and D.F. has a PhD fellowship (FPU-AP2009-3736). S.R. is funded by the Laboratoire d’Excellence (LABEX) TULIP (ANR-10-LABX-41). M.-P.G.-G. received a “Juan de la Cierva” postdoctoral contract from the Spanish Ministry of Science in the Ana Caño (A.I.C.-D.) laboratory, and an HFSP short-term fellowship in the Benfey (P.N.B.) laboratory. P.N.B. is funded by NSF Arabidopsis 2010 grant. Work in the Ana Caño (A.I.C.-D.) laboratory is funded by a BIO2010/007 grant from the Spanish Ministry of Innovation and Science and a Marie-Curie Initial Training Network “BRAVISSIMO” (grant no. PITN-GA-2008-215118).
- Published
- 2014
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