1. Adjuvant effect in aquaculture fish of cell-wall glycolipids isolated from acid-fast bacteria
- Author
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Hideki Kuriyama, Megumi Matsumoto, Yutaka Takeuchi, Sayaka Nishimura, Atsushi Yamamoto, Teruyuki Nakanishi, Kyosuke Araki, and Kazuhiro Shiozaki
- Subjects
0301 basic medicine ,Carps ,medicine.medical_treatment ,Immunology ,Nocardia Infections ,Aquaculture ,Microbiology ,03 medical and health sciences ,Fish Diseases ,Glycolipid ,Immune system ,Adjuvants, Immunologic ,Immunity ,medicine ,Animals ,Amberjack ,Vaccines ,biology ,Bacteria ,04 agricultural and veterinary sciences ,Th1 Cells ,biology.organism_classification ,Up-Regulation ,carbohydrates (lipids) ,Survival Rate ,030104 developmental biology ,040102 fisheries ,Crucian carp ,0401 agriculture, forestry, and fisheries ,Cytokines ,lipids (amino acids, peptides, and proteins) ,Glycolipids ,Adjuvant ,Developmental Biology ,Mycobacterium - Abstract
Mycobacteriosis and nocardiosis in cultured fish caused by infections with acid-fast bacteria, are responsible for large economic losses globally. In this study, we suggest a novel adjuvant using glycolipids that activates host immune systems. The immune response to glycolipids stimulation was investigated using ginbuna crucian carp. Ginbuna vaccinated with FKC (formalin-killed cells) + glycolipids isolated from Mycobacterium sp., upregulated inflammatory- and Th1-related cytokines, and a DTH (delayed-type hypersensitivity) response was confirmed only in ginbuna vaccinated with FKC + glycolipids. These observations suggest that glycolipids activated host innate and cell-mediated immunity. Subsequently, we evaluated the adjuvant effect of glycolipids against amberjack nocardiosis. In a challenge test, a higher survival rate was observed in amberjack vaccinated with FKC + glycolipids emulsified with conventional oil adjuvant than in fish vaccinated with FKC + oil adjuvant without glycolipids. Therefore, glycolipids potentially could be used as a practical, economical and safe adjuvant for aquaculture fish.
- Published
- 2018