1. Transcriptional regulation of type I interferon gene expression by interferon regulatory factor-3 in Japanese flounder, Paralichthys olivaceus
- Author
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Ohtani, Maki, Hikima, Jun-ichi, Hwang, Seong Don, Morita, Takahiro, Suzuki, Yoshiaki, Kato, Goshi, Kondo, Hidehiro, Hirono, Ikuo, Jung, Tae-Sung, and Aoki, Takashi
- Subjects
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INTERFERON genetics , *GENE expression in fishes , *PARALICHTHYS , *GENETIC transcription , *INTERFERON regulatory factors , *TOLL-like receptors , *FISH immunology - Abstract
Abstract: Type I interferon (IFN) induces the antiviral response in innate immunity. The type I IFN gene cloned from Japanese flounder (Paralichthys olivaceus) has a length of 1189bp and consisting of 5 exons and 4 introns. In a phylogenetic tree of type I IFNs, Japanese flounder grouped with other Acanthopterygii. To gain insight into the transcriptional regulation of IFN gene, the 1.36kb 5′-upstream region including numerous canonical motifs to bind transcription factors [for example, IFN regulatory factor (IRF)] was analyzed. In HINAE cells using a luciferase reporter assay, poly I:C-responsive transcriptional activity was found in the region from −634 to −179bp. This region includes several IRF motifs. In the presence of poly I:C, overexpression of IRF3 and RLR strongly enhanced transcriptional activity. These results suggest that the transcriptional regulation of Japanese flounder type I IFN is regulated by IRF3 after triggering with dsRNA sensors. [Copyright &y& Elsevier]
- Published
- 2012
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