1. In vitro study on the osteogenesis enhancement effect of BMP-2 incorporated biomimetic apatite coating on titanium surfaces
- Author
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Xinchun Zhang, Xiaojing Zhu, Chengyun Ning, Hui Zhang, and Yan Wang
- Subjects
animal structures ,Materials science ,Dentistry ,chemistry.chemical_element ,02 engineering and technology ,engineering.material ,Calcium ,Bone morphogenetic protein ,Bone morphogenetic protein 2 ,Apatite ,03 medical and health sciences ,0302 clinical medicine ,Coating ,General Dentistry ,Supersaturation ,business.industry ,030206 dentistry ,021001 nanoscience & nanotechnology ,In vitro ,Chemical engineering ,chemistry ,visual_art ,embryonic structures ,Ceramics and Composites ,engineering ,visual_art.visual_art_medium ,0210 nano-technology ,business ,Titanium - Abstract
To fabricate a sustained-release delivery system of bone morphogenetic protein (BMP-2) on titanium surface, explore the effect of BMP-2 concentration on the loading/release behavior of BMP-2 and evaluate the cell compatibility of the system in vitro, pure titanium specimens were immersed into supersaturated calcium phosphate solutions (SCP) containing 4 different concentrations of BMP-2: 0, 50, 100, 200 and 400 ng/mL. Biomimetic calcium phosphate coating was formed on titanium surface and BMP-2 was incorporated into the coating through co-deposition. The release profile of BMP-2 suggested that BMP-2 were delivered sustainably up to 20 days. CCK-8 and ALP assay showed that 200 group and 400 ng/mL BMP-2 group have significant effect on promoting MC3T3-E1 cell proliferation and differentiation. The BMP-2 incorporated into the hybrid coating released in a sustained manner and significantly promoted the proliferation and differentiation of MC3T3-E1 on the titanium surface.
- Published
- 2017
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