The aim of this study was to produce activated carbon derived from corn stalk (AC-CS) with suitable characteristics as inexpensive, nontoxic adsorbent with good efficiency for elimination of Methylene Blue (MB) as cationic dye from aqueous solution in batch adsorption process. The morphology and functional groups of adsorbent were characterized by SEM and FTIR in this dataset. In addition, the influence of MB concentration, pH, adsorbent dosage, and contact time on the removal of dye using AC-CS was tested by central composite design (CCD) under response surface methodology (RSM). Based on results, the parameters adsorbent dose and initial dye concentration for this investigation play an important role in the adsorption studies of methylene blue. The experimental values were in good agreement with the model predicted values also the results of the study showed that maximum absorbance efficiency at initial concentration of 10 mg/l, absorbent dose of 1.4 g, contact time of 50 min and pH 11 was 90%. Keywords: Methylene blue, Adsorption, Response surface methodology, Waste corn