1. Identification of pig chromosomes in pig-mouse somatic cell hybrid bivariate flow karyotypes.
- Author
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Bouvet A, Konfortov BA, Miller NG, Brown D, and Tucker EM
- Subjects
- Animals, Bisbenzimidazole, Chromomycin A3, Chromosome Banding, DNA analysis, Female, Flow Cytometry, Male, Mice genetics, Species Specificity, Chromosomes ultrastructure, Hybrid Cells ultrastructure, Karyotyping methods, Swine genetics
- Abstract
To identify pig chromosomes in pig-mouse somatic cell hybrids, dual-laser flow karyotypes and GTG-banded metaphase spreads of pig, mouse, and 7 pig-mouse hybrid cell lines were compared. Pig chromosomes no. 1, 2, 5, 6, 10, 11, 13, 14, 16, 18, X and Y were tentatively assigned to individual peaks in the pig flow karyotype on the basis of DNA content vs. relative chromosome length. In the 7 hybrid cell lines, 7 out of 8 peaks distinct from those of the mouse cell line could be correlated with the presence of pig chromosomes no. 5, 9, 10, 11 or 16, 14, 15, and 18, whereas 1 peak appeared to correspond to the presence of 1 middle-size chromosome (3, 4, or 7). Other pig chromosomes present in the hybrids could not be detected with certainty due to superposition with mouse peaks and mouse chromosome rearrangements.
- Published
- 1993
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