1. Higher circulating levels of chemokines CXCL10, CCL20 and CCL22 in patients with ischemic heart disease
- Author
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Mohammad Mahdi Mohammadi, M. Khalili, Abdollah Jafarzadeh, Hamidreza Rashidinejad, Maryam Nemati, Shahriar Dabiri, and Amin Safa
- Subjects
0301 basic medicine ,Adult ,Male ,medicine.medical_specialty ,Statin ,medicine.drug_class ,Immunology ,Myocardial Ischemia ,Single-nucleotide polymorphism ,Biochemistry ,Gastroenterology ,Polymorphism, Single Nucleotide ,Pathogenesis ,03 medical and health sciences ,Internal medicine ,Genotype ,medicine ,Immunology and Allergy ,Humans ,Myocardial infarction ,Molecular Biology ,Chemokine CCL22 ,Aspirin ,Chemokine CCL20 ,biology ,Unstable angina ,business.industry ,Angiotensin-converting enzyme ,Hematology ,Middle Aged ,medicine.disease ,Chemokine CXCL10 ,030104 developmental biology ,biology.protein ,Female ,business ,medicine.drug - Abstract
Recruitment of leukocytes is one of the earliest events in the pathogenesis of ischemic heart disease (IHD) and chemokines play an important role in the migration of these cells into the inflammation sites. The aim of this study was to evaluate the CXCL10, CCL20 and CCL22 levels and the single nucleotide polymorphisms (SNPs) rs4508917, rs6749704 and rs4359426 in chemokine genes in patients with IHD to clarify any association. A total of 300 patients with IHD as having acute myocardial infarction (AMI; n=100), stable angina (SA; n=100) or unstable angina (UA; n=100) and 100 healthy subjects as a control group were enrolled to study. Serum samples from all participants were tested for the CXCL10, CCL20 and CCL22 levels by using ELISA. The SNPs were determined by polymerase chain reaction-restriction length polymorphism (PCR-RFLP) method. The mean serum concentrations of CXCL10, CCL20 and CCL22 in AMI patients (395.97±21.20Pg/mL, 108.38±10.31Pg/mL and 1852.58±205.77Pg/mL), SA patients (405.48±27.36Pg/mL, 90.20±7.69Pg/mL and 2322.04±231.23Pg/mL) and UA patients (396.69±22.79Pg/mL, 141.87±18.10Pg/mL and 2754.89±211.70Pg/mL) were significantly higher than in the healthy group (179.38±8.85Pg/mL, 51.92±4.62Pg/mL and 451.82±23.76Pg/mL, respectively; P
- Published
- 2016