1. The Th1/Th2/Th17 cytokine profile of HIV-infected individuals: a multivariate cytokinomics approach
- Author
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Francois E. Steffens, Debra Meyer, Carolus J. Reinecke, and Aurelia Alvina Williams
- Subjects
Adult ,Male ,Multivariate statistics ,medicine.medical_specialty ,cytokinomics ,Cytokine profile ,Immunology ,ComputingMilieux_LEGALASPECTSOFCOMPUTING ,HIV Infections ,Biology ,Cytometric bead array ,Biochemistry ,Young Adult ,multivariate ,Th2 Cells ,Hiv infected ,Agency (sociology) ,ComputingMilieux_COMPUTERSANDEDUCATION ,medicine ,Immunology and Allergy ,Humans ,Technology innovation ,Molecular Biology ,interleukin ,Interleukin-6 ,flow cytometry ,Interleukin-17 ,Discriminant Analysis ,Hematology ,T-Lymphocytes, Helper-Inducer ,Th1 Cells ,Medical research ,Tissue Donors ,Interleukin-10 ,ComputingMilieux_GENERAL ,Logistic Models ,Family medicine ,Multivariate Analysis ,Cytokines ,Th17 Cells ,Female - Abstract
HIV infection causes the dysregulation of cytokine production. A cytokinomics approach employing cytometric bead array (CBA) technology, flow cytometry and multivariate analysis was applied to the investigation of HIV-induced T helper cell type 1 (Th1), Th2 and Th17 cytokine changes in the sera of treatment naive individuals. Stepwise linear discriminant analysis (LDA) and logistic regression identified interleukin (IL)-6 to be discriminatory for HIV infection with 74.6% and 71.2% of the cases correctly classified. Analysis of variance (ANOVA) confirmed IL-6 and IL-10 concentrations to be significantly (p = 0.001 and p = 0.025) different between the groups. A scatter plot of the log IL-6 and IL-10 concentrations for the groups largely overlapped, with improved differentiation where patients were advancing to the acquired immunodeficiency syndrome (AIDS). IL-17A levels were higher than other cytokines but did not significantly distinguish the groups suggesting that the HIV and HIV+ individuals had similar immune profiles. This possibility was supported by other clinical indicators. Taken together, the measured cytokines (IL-6, 10 and 17) have potential prognostic value. http://www.journals.elsevier.com/cytokine/
- Published
- 2012