1. Impact of Zika virus on the human type I interferon osteoimmune response.
- Author
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Drouin A, Wallbillich N, Theberge M, Liu S, Katz J, Bellovoda K, Se Yun Cheon S, Gootkind F, Bierman E, Zavras J, Berberich MJ, Kalocsay M, Guastaldi F, Salvadori N, Troulis M, and Fusco DN
- Subjects
- Animals, Antiviral Agents pharmacology, Cell Line, Tumor, Chlorocebus aethiops, Gene Expression Regulation drug effects, Gene Expression Regulation immunology, Host-Pathogen Interactions drug effects, Host-Pathogen Interactions immunology, Humans, Interferon Type I pharmacology, Mice, Knockout, Osteoblasts metabolism, Osteoblasts virology, Proteome immunology, Proteome metabolism, Proteomics methods, Vero Cells, Virus Replication drug effects, Virus Replication immunology, Zika Virus physiology, Zika Virus Infection metabolism, Zika Virus Infection virology, Mice, Antiviral Agents immunology, Interferon Type I immunology, Osteoblasts immunology, Zika Virus immunology, Zika Virus Infection immunology
- Abstract
Background: The developing field of osteoimmunology supports importance of an interferon (IFN) response pathway in osteoblasts. Clarifying osteoblast-IFN interactions is important because IFN is used as salvage anti-tumor therapy but systemic toxicity is high with variable clinical results. In addition, osteoblast response to systemic bursts and disruptions of IFN pathways induced by viral infection may influence bone remodeling. ZIKA virus (ZIKV) infection impacts bone development in humans and IFN response in vitro. Consistently, initial evidence of permissivity to ZIKV has been reported in human osteoblasts., Hypothesis: Osteoblast-like Saos-2 cells are permissive to ZIKV and responsive to IFN., Methods: Multiple approaches were used to assess whether Saos-2 cells are permissive to ZIKV infection and exhibit IFN-mediated ZIKV suppression. Proteomic methods were used to evaluate impact of ZIKV and IFN on Saos-2 cells., Results: Evidence is presented confirming Saos-2 cells are permissive to ZIKV and support IFN-mediated suppression of ZIKV. ZIKV and IFN differentially impact the Saos-2 proteome, exemplified by HELZ2 protein which is upregulated by IFN but non responsive to ZIKV. Both ZIKV and IFN suppress proteins associated with microcephaly/pseudo-TORCH syndrome (BI1, KI20A and UBP18), and ZIKV induces potential entry factor PLVAP., Conclusions: Transient ZIKV infection influences osteoimmune state, and IFN and ZIKV activate distinct proteomes in Saos-2 cells, which could inform therapeutic, engineered, disruptions., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2021
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