1. New developments in automated cytogenetic imaging: unattended scoring of dicentric chromosomes, micronuclei, single cell gel electrophoresis, and fluorescence signals.
- Author
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Schunck C, Johannes T, Varga D, Lörch T, and Plesch A
- Subjects
- Animals, Automation, Cell Count instrumentation, Cell Nucleus ultrastructure, Chromosome Aberrations, Comet Assay instrumentation, DNA Damage, Equipment Design, Gamma Rays, Humans, Lymphocytes radiation effects, Lymphocytes ultrastructure, Microcomputers, Micronucleus Tests instrumentation, Microscopy instrumentation, Microscopy, Fluorescence instrumentation, Microscopy, Fluorescence methods, Mutagenicity Tests instrumentation, Radiometry instrumentation, Radiometry methods, Reproducibility of Results, Cytogenetic Analysis instrumentation, Image Processing, Computer-Assisted instrumentation, Microscopy methods
- Abstract
The quantification of DNA damage, both in vivo and in vitro, can be very time consuming, since large amounts of samples need to be scored. Additional uncertainties may arise due to the lack of documentation or by scoring biases. Image analysis automation is a possible strategy to cope with these difficulties and to generate a new quality of reproducibility. In this communication we collected some recent results obtained with the automated scanning platform Metafer, covering applications that are being used in radiation research, biological dosimetry, DNA repair research and environmental mutagenesis studies. We can show that the automated scoring for dicentric chromosomes, for micronuclei, and for Comet assay cells produce reliable and reproducible results, which prove the usability of automated scanning in the above mentioned research fields., (Copyright 2003 S. Karger AG, Basel)
- Published
- 2004
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