1. Comparative analysis of the region of the mitochondrial genome contaning the ATPase subunit 9 gene in the two related yeast species Saccharomyces douglasii and Saccharomyces cerevisiae
- Author
-
Paolo Laveder, Laura Nicoletti, Rossella Pellizzari, Giovanna Carignani, and Barbara Cardazzo
- Subjects
Mitochondrial DNA ,Transcription, Genetic ,Genes, Fungal ,Molecular Sequence Data ,Saccharomyces cerevisiae ,Sequence Homology ,DNA, Mitochondrial ,Conserved sequence ,Fungal Proteins ,Saccharomyces ,Intergenic region ,Species Specificity ,Genetics ,Coding region ,Amino Acid Sequence ,RNA Processing, Post-Transcriptional ,DNA, Fungal ,Gene ,Adenosine Triphosphatases ,Base Composition ,Base Sequence ,biology ,Nucleic acid sequence ,General Medicine ,biology.organism_classification ,Regulatory sequence ,Sequence Alignment - Abstract
We have determined the nucleotide sequence of a region of the mitochondrial genome of the yeast Saccharomyces douglasii which contains the ATPase subunit 9 gene and part of the intergenic sequences that surround it. The gene is 228 nucleotides long and encodes a polypeptide of 76 aa. A comparison of the coding sequence with that of S. cerevisiae reveals the presence of three silent transitions. A high level of similarity is also found between regions involved in the initiation of transcription and mRNA processing. More interestingly, a region of similarity situated outside the known regulatory regions has been identified. As the intergenic regions are generally highly divergent, the remarkable conservation of these non-coding sequences suggests that their structure may be relevant to the expression of this region of the mitochondrial DNA.
- Published
- 1994