1. Phospho-mimicking Atf1 mutants bypass the transcription activating function of the MAP kinase Sty1 of fission yeast.
- Author
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Sánchez-Mir, Laura, Salat-Canela, Clàudia, Paulo, Esther, Carmona, Mercè, Ayté, José, Oliva, Baldo, and Hidalgo, Elena
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MITOGEN-activated protein kinases , *PHOSPHORYLATION , *TRANSCRIPTION factors , *GENETIC regulation , *GENE expression - Abstract
Stress-dependent activation of signaling cascades is often mediated by phosphorylation events, but the exact nature and role of these phosphorelays are frequently poorly understood. Here, we review which are the consequences of the stress-dependent phosphorylation of a transcription factor on gene activation. In fission yeast, the MAP kinase Sty1 is activated upon several environmental hazards and promotes cell adaptation and survival, greatly through activation of a gene program mediated by the transcription factor Atf1. Although described decades ago, the role of the phosphorylation of Atf1 by Sty1 is still a matter of debate. We present here a brief review of recent data, obtained through the characterization of several phosphorylation mutant derivatives of Atf1, demonstrating that Atf1 phosphorylation does not stabilize the factor nor stimulates its binding to DNA. Rather, it provides a structural platform of interaction with the transcriptional machinery. Based on these findings, future work will establish how this phosphorylated trans-activation domain promotes the massive gene expression shift allowing cellular adaptation to stress. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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