1. Survival of canine kidneys after treatment with dimethyl-sulfoxide, freezing at −80 °C, and thawing by microwave illumination
- Author
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Claire Turgeon-Knaack, Hervé Blanchard, Jacques Lizin, Pierre Robitaille, and Frank M. Guttman
- Subjects
medicine.medical_specialty ,Chromatography ,Chemistry ,Dimethyl sulfoxide ,Organ Preservation ,General Medicine ,Kidney ,Kidney Transplantation ,Transplantation, Autologous ,Single kidney ,General Biochemistry, Genetics and Molecular Biology ,Surgery ,Perfusion ,chemistry.chemical_compound ,Dogs ,Freezing ,Toxicity ,medicine ,Animals ,Dimethyl Sulfoxide ,Tissue Preservation ,Microwaves ,General Agricultural and Biological Sciences ,After treatment - Abstract
Forty canine kidneys were the subject of this pilot study where control groups perfused with Perfudex plus DMSO (1.4 m ), modified Collins' solution with DMSO (1.4 m ) and modified Sacks' solution with DMSO (1.4 m ) showed little toxicity and life-sustaining conservation. In the experimental group, 16 kidneys were frozen for 15 min to −80 °C, thawed by microwave illumination, and reimplanted. Of the 16 dogs, eight survived 2–14 months on their single kidney. The technique of inducing freezing by using intra-arterial cold helium and thawing with high-power microwave illumination gave an overall success rate of 50% long-term life-sustaining survival.
- Published
- 1977
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