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Increasing seed yield and quality of key cool-season perennial grasses is an important component of meeting China's environmental, food security, and urban beautification goals. Determining a suitable seed producing region and developing best management practices for this region with specific, high priority grasses will contribute to the availability of seed for reducing desertification, increasing livestock production, and improving urban living conditions. Previous research in other areas has addressed various agronomic practices on some grasses, but none has evaluated optimal row spacings for producing high yields of high quality seed in conditions similar to the Hexi Corridor, currently a major seed producing area in China for other seed crops. We compared our field trials in this area with those of other regions in China and the world to determine if this area would be suitable as a grass seed production center. Five important perennial, cool-season grasses {[Elymus kamoji (ohwi) S.L. Chen, slender wheatgrass [Elymus trachycaulus (Link) Gould ex Shinners ssp. Trachycaulus], smooth bromegrass (Bromus inermis Leyss), Siberian wildrye (Elymus sibiricus L.), and Chinese sheepgrass [Leymus chinensis (Trin) Tzvel]} were evaluated from 2010 to 2012 under four row spacing treatments (30, 50, 70, and 90 cm). Results showed all five grasses have potential for high seed production in this region, with yields equivalent to or significantly higher than other areas. Optimal row spacing for Elymus kamoji and smooth bromegrass was 30 cm while a 50 cm row spacing was better for seed production and weed control for slender wheatgrass, Siberian wildrye, and Chinese sheepgrass. This work will allow multinational seed companies, development agencies, and government program directors to better evaluate the potential economic sustainability of grass seed production in this region compared to importing seed from other regions of the world. [ABSTRACT FROM AUTHOR]