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The matrix metalloproteinases (MMPs) participate in fundamental processes, such as cell proliferation, differentiation, adhesion, migration, angiogenesis, apoptosis, and inflammation [1]. The increased expression of MMPs suggests that these proteases may influence the pathogenesis of endotoxemia in sepsis [2]. The objective of this study was to evaluate the serum activity of MMP-2 and MMP-9, length of hospital stay, length of ICU stay and mortality in patients with septic shock.

Severe sepsis and septic shock are major healthcare problems, affecting millions of people around the world each year, and increasing in incidence [1]. During sepsis there is increased oxidative stress, with reduced body stores of selenium (Se) and lower activity of glutathione peroxidase (GPx1), with patient outcomes of multiple organ failure and death [2]. The objective of this study was to determine the influence of Se concentration in plasma, erythrocytes and erythrocyte GPx1 activity on the length of hospital stay, length of ICU stay and ICU mortality in patients with septic shock.

Thiamine deficiency can be present in 20% of patients in the ICU. This deficiency is considered to be an uncommon source of lactic acidosis in septic patients. An elevated serum lactate level is associated with morbidity and mortality [1]. Increased levels of thiamine increase the activity of glutathione peroxidase (GPx), a major component of the cellular antioxidant system [2]. The objective of this study was to determine the influence of serum thiamine concentrations on lactate levels, GPx activity, length of hospital stay, length of ICU stay and ICU mortality in patients with septic shock.

Made available in DSpace on 2015-03-18T15:55:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-01-01Bitstream added on 2015-03-18T16:28:32Z : No. of bitstreams: 1 WOS000341163800006.pdf: 236353 bytes, checksum: c1c2c25bd98b5dfe38d2a0924d7c5e75 (MD5) Made available in DSpace on 2015-03-18T15:55:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-01-01Bitstream added on 2015-03-18T16:28:32Z : No. of bitstreams: 1 WOS000341163800006.pdf: 236353 bytes, checksum: c1c2c25bd98b5dfe38d2a0924d7c5e75 (MD5) Made available in DSpace on 2015-03-18T15:55:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-01-01Bitstream added on 2015-03-18T16:28:32Z : No. of bitstreams: 1 WOS000341163800006.pdf: 236353 bytes, checksum: c1c2c25bd98b5dfe38d2a0924d7c5e75 (MD5) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Introduction: Selenoenzymes can modulate the extent of oxidative stress, which is recognized as a key feature of septic shock. The pathophysiologic role of erythrocyte selenium concentration in patients with septic shock remains unknown. Therefore, the objective of this study was to evaluate the association of erythrocyte selenium concentration with glutathione peroxidase (GPx1) activity, GPx1 polymorphisms and with ICU and hospital mortality in septic shock patients.Methods: This prospective study included all patients older than 18 years with septic shock on admission or during their ICU stay, admitted to one of the three ICUs of our institution, from January to August 2012. At the time of the patients' enrollment, demographic information was recorded. Blood samples were taken within the first 72 hours of the patients' admission or within 72 hours of the septic shock diagnosis for determination of selenium status, protein carbonyl concentration, GPx1 activity and GPx1 Pro198Leu polymorphism (rs 1050450) genotyping.Results: A total of 110 consecutive patients were evaluated. The mean age was 57.6 +/- 15.9 years, 63.6% were male. Regarding selenium status, only erythrocyte selenium concentration was lower in patients who died in the ICU. The frequencies for GPx1 Pro198Leu polymorphism were 55%, 38% and 7% for Pro/Pro, Pro/Leu and Leu/Leu, respectively. In the logistic regression models, erythrocyte selenium concentration was associated with ICU and hospital mortality in patients with septic shock even after adjustment for protein carbonyl concentration and acute physiology and chronic health evaluation II score (APACHE II) or sequential organ failure assessment (SOFA).Conclusions: Erythrocyte selenium concentration was a predictor of ICU and hospital mortality in patients with septic shock. However, this effect was not due to GPx1 activity or Pro198Leu polymorphism. UNESP Univ Estadual Paulista, Dept Internal Med, Botucatu Med Sch, BR-18618970 Sao Paulo, Brazil Univ Sao Paulo, Dept Food & Expt Nutr, Fac Pharmaceut Sci, BR-05508070 Sao Paulo, Brazil UNESP Univ Estadual Paulista, Chem & Biochem Dept, Inst Biol Sci, BR-18618970 Sao Paulo, Brazil UNESP Univ Estadual Paulista, Dept Internal Med, Botucatu Med Sch, BR-18618970 Sao Paulo, Brazil UNESP Univ Estadual Paulista, Chem & Biochem Dept, Inst Biol Sci, BR-18618970 Sao Paulo, Brazil