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3 chemically pure fractions were isolated from Napp printing plate and subsequently identified by means of nuclear magnetic resonance, infrared spectrophotometry and elemental analysis. 1 of the fractions elicited positive test reactions in 3 Napp-allergic printers, and another fraction also elicited a positive test reaction in 1 of the printers. The 2 allergens were 2-hydroxyethyl methacrylate and N,N'-methylene-bis-acrylamide. The 1st of the 2 allergens was also demonstrated in another printing plate, Nyloprint WD.

Two male employees in an industry producing plastic emulsions acquired contact dermatitis from preservatives. One was sensitized to both components in a preservative, i.e. 1,2-benzisothiazolin-3-one and ethylene diamine. The other man acquired a contact allergy to 1,2-benzisothiazolin-3-one and to another preservative, formaldehyde.

Several chemically pure fractions have been isolated from Nyloprint printing plates. 5 of the fractions elicited positive patch test reactions in 7 Nylonprint allergic printers. 4 of the 5 components were analysed by means of nuclear magnetic resonance, infrared spectrophotometry, mass spectrometry and elemental analysis. The fractions were identified as N,N'-methylene-bis-acrylamide. N,N'-[oxybis(methylene)]bis-2-propenamide, N,N'-[1,2-ethanediylbis(oxymethylene)]-2-propenamide, and a new compound N-[(2-hydroxyethoxy)-methyl]-2-propenamide. All of the analysed fractions have the common feature of being secondary acrylamides.