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Blue ling is a deep-water species that has been severely fished upon into coastal and offshore fisheries since the early 1970s, thus causing the collapse of the populations in the last two decades. Genetic information is scarce in this species, and molecular markers are therefore needed to provide advice both for management and for rebuilding the stocks. A suite of 103 SNP markers was identified from ddRAD sequencing data. From those, 81 were organized in three multiplex reactions, and tested on 150 individuals from three different sampling locations. Good-quality amplification products were successfully obtained from 70 of the markers. All SNP loci were biallelic, with averaged He per locus ranging between 0.101 and 0.500.

We developed and characterized 20 microsatellite primer loci for the northern shrimp Pandalusborealis. All 20 loci were polymorphic with number of alleles ranging from 3 to 36 and with observed heterozygosity between 0.04 and 0.93. In addition, we tested the utility of these markers in three related shrimp species, P. montagui, Atlantopandaluspropinqvus and Dichelopandalusbonnieri. These new markers will prove useful in the identification of stock structure and hence, assessment of the commercially important species P. borealis.

Fourteen polymorphic microsatellite markers with tetranucleotide repeats were developed for the bearded goby (Sufflogobius bibarbatus) from partial genomic DNA libraries using a repeat enrichment protocol, and characterized using two putative populations from the northern Benguela. The average number of alleles per locus ranged from 4 to 34, and the observed heterozygosities across loci were between 0.237 and 0.983. We also tested the utility of these markers in two other marine gobies; sand goby Pomatoschistus minutus and two-spotted goby Gobiusculus flavescens. These polymorphic markers can be employed to investigate population structure and related questions of the bearded goby.