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The sympathetic nervous system (SNS) is essential for the body's immediate response to stress, initiating physiological changes that can be measured through sympathetic nerve activity (SNA). While microneurography (MNG) is the gold standard for direct SNA measurement, its invasive nature limits its practical use in clinical settings. This study investigates the use of multi-wavelength photoplethysmography (PPG) as a non-invasive alternative for SNA measurement. Key features are extracted from the pulsatile components of red and green PPG signals to train a linear regression machine learning (ML) model to predict R-wave-triggered spike count (SPR), a biomarker derived from MNG. The study correlates PPG-derived features with ground truth SPR to develop a predictive model capable of detecting SNA during induced physical stress (isometric handgrip and cold pressor) and cognitive stress (mental arithmetic and Stroop test). Unlike previous research that relies on subjective stress indicators, our work utilizes MNG-derived SPR as an objective ground truth for validation. Our findings demonstrate strong agreement between PPG-predicted SPR values and those obtained via MNG, with red PPG showing a higher correlation. The green wavelength PPG exhibits greater sensitivity in detecting stress-induced SNA, particularly during stress onset, where it outperforms the MNG method in capturing immediate responses to stressors such as mental arithmetic and the cold pressor task. To the best of our knowledge, this is the first study to directly compare PPG-derived SNA estimates with MNG, offering a promising pathway for developing wearable, non-invasive tools for continuous stress monitoring and sympathetic arousal detection., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Ltd.)

Squamous cell carcinoma is a prevalent cancer type that affects various organs in the human body. Manual analysis for detecting squamous cell carcinoma in histopathological images is time-consuming and may be subjective. Squamous cell carcinoma diagnosis is typically based on the differences in the architectural arrangement of squamous epithelial layers and the presence of keratinization. However, the existing literature has predominantly concentrated on identifying cellular irregularities with high magnification images and considering specific organs of squamous cell carcinoma origin. In contrast, relatively little attention has been given to recognizing structural abnormalities observable at low magnification images. In this paper, we consider squamous cell carcinoma histopathological images across different organs of origin captured at low magnification and these images are gathered from various centers to develop a robust model. We propose a novel deep neural network model (MultiSCCHisto-Net) that can detect squamous cell carcinoma of any organ irrespective of organ of origin. In addition, deep neural networks used for histopathological image analysis typically have many parameters, making them computationally expensive. To address this research gap, we incorporate knowledge distillation, which compresses knowledge from a complex teacher model (MultiSCCHisto-Net) into a smaller student model (MultiSCCHisto-Net-KD) while preserving performance and enhancing the generalization of the student model by learning from the teacher's intermediate layer features. Moreover, an explainable deep learning technique called gradient-weighted class activation mapping is incorporated to highlight the image areas that help to classify the sample into particular classes. This explainability significantly enhances our confidence in the proposed model outcomes. We evaluate the model's robustness using private multi-centric and publicly available datasets. Our results show that accuracy rates of 97% and 93% are achieved on private and public datasets, respectively, surpassing the performance of state-of-the-art models., Competing Interests: Declaration of competing interest The authors have no conflicts of interest to declare. All co-authors have seen and agree with the contents of the manuscript and there is no financial interest to report. We certify that the submission is original work and is not under review at any other publication., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Diabetes mellitus (DM) represents a major global health challenge, affecting a diverse range of demographic populations across all age groups. It has particular implications for women during pregnancy and the postpartum period. The contemporary prevalence of sedentary lifestyle patterns and suboptimal dietary practices has substantially contributed to the escalating incidence of this metabolic disorder. The timely identification of diabetes mellitus (DM) in the female population is crucial for preventing related complications and facilitating the implementation of effective therapeutic interventions. However, conventional predictive models frequently demonstrate limited external validity when applied across heterogeneous datasets, potentially compromising clinical utility. This study proposes a robust machine learning (ML) framework for diabetes prediction across diverse populations using two distinct datasets: the PIMA and BD datasets. The framework employs intra-dataset, inter-dataset, and partial fusion dataset validation techniques to comprehensively assess the generalizability and performance of various models. In intra-dataset validation, the Extreme Gradient Boosting (XGBoost) model achieved the highest accuracy on the PIMA dataset with 79%. In contrast, the Random Forest (RF) and Gradient Boosting (GB) models demonstrated accuracy close to 99% on the BD dataset. For inter-dataset validation, where models were trained on one dataset and tested on the other, the ensemble model outperformed others with 88% accuracy when trained on PIMA and tested on BD. However, model performance declined when trained on BD and tested on PIMA (74%), reflecting the challenges of inter-dataset generalization ability. Finally, during partial fusion data validation, the deep learning (DL) model achieved 74% accuracy when trained on the BD dataset augmented with 30% of the PIMA dataset. This accuracy increased to 98% when training on the PIMA dataset combined with 30% of the BD data. These findings emphasize the importance of dataset diversity and the partial fusion dataset that can significantly enhance the model's robustness and generalizability. This framework offers valuable insights into the complexities of diabetes prediction across heterogeneous environments., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2025 The Authors. Published by Elsevier Ltd.. All rights reserved.)

The intersection of Artificial Intelligence (AI) and perinatal mental health research presents promising avenues, yet uncovers significant challenges for innovation. This review explicitly focuses on this multidisciplinary field and undertakes a comprehensive exploration of existing research therein. Through a scoping review guided by the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) framework, we searched relevant literature spanning a decade (2013-2023) and selected fourteen studies for our analysis. We first provide an overview of the main AI techniques and their development, including traditional methods across different categories, as well as recent emerging methods in the field. Then, through our analysis of the literature, we summarize the predominant AI and ML techniques adopted and their applications in perinatal mental health studies, such as identifying risk factors, predicting perinatal mental health disorders, voice assistants, and Q&A chatbots. We also discuss existing limitations and potential challenges that hinder AI technologies from improving perinatal mental health outcomes, and suggest several promising directions for future research to meet real needs in the field and facilitate the translation of research into clinical settings., Competing Interests: Declaration of competing interest None Declared., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Research evidence shows that physical rehabilitation exercises prescribed by medical experts can assist in restoring physical function, improving life quality, and promoting independence for physically disabled individuals. In response to the absence of immediate expert feedback on performed actions, developing a Human Action Evaluation (HAE) system emerges as a valuable automated solution, addressing the need for accurate assessment of exercises and guidance during physical rehabilitation. Previous HAE systems developed for the rehabilitation exercises have focused on developing models that utilize skeleton data as input to compute a quality score for each action performed by the patient. However, existing studies have focused on improving scoring performance while often overlooking computational efficiency. In this research, we propose LightPRA (Light Physical Rehabilitation Assessment) system, an innovative architectural solution based on a Temporal Convolutional Network (TCN), which harnesses the capabilities of dilated causal Convolutional Neural Networks (CNNs). This approach efficiently captures complex temporal features and characteristics of the skeleton data with lower computational complexity, making it suitable for real-time feedback provided on resource-constrained devices such as Internet of Things (IoT) devices and Edge computing frameworks. Through empirical analysis performed on the University of Idaho-Physical Rehabilitation Movement Data (UI-PRMD) and KInematic assessment of MOvement for remote monitoring of physical REhabilitation (KIMORE) datasets, our proposed LightPRA model demonstrates superior performance over several state-of-the-art approaches such as Spatial-Temporal Graph Convolutional Network (STGCN) and Long Short-Term Memory (LSTM)-based models in scoring human activity performance, while exhibiting lower computational cost and complexity., Competing Interests: Declaration of competing interest The authors whose names are listed immediately below certify that they have NO affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

In the study of tumor disease pathogenesis, the identification of genes specifically expressed in disease states is pivotal, yet challenges arise from high-dimensional datasets with limited samples. Conventional gene (feature) selection methods often fall short of capturing the complexity of gene-phenotype and gene-gene interactions, necessitating a more robust analysis method. To address these challenges, a gene subset augmentation strategy is proposed in this paper. Our approach introduces diverse perturbation mechanisms to generate distinct gene subsets. The partial least squares-based multiple gene measurement algorithm considers gene-phenotype and gene-gene correlations, identifying differentially expressed genes, including those with weak signals. The constructed gene networks derived from the augmented subsets unveil regulatory patterns, enabling association analysis to explore gene associations comprehensively. Our algorithm excels in identifying small-sized gene subsets with strong discriminative power, surpassing traditional methods that yield a single gene subset. Unlike conventional approaches, our algorithm reveals a spectrum of different gene subsets and their weakly differentially expressed genes. This nuanced perspective aids in unraveling the molecular characteristics and specific expression patterns of tumor genes. The versatility of our approach not only contributes to the advancement of tumor-specific gene identification but also holds promise for addressing challenges in various fields characterized by high-dimensional datasets and limited samples. The Python implementation is available at http://github.com/wenjieyou/PLSGSA., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Cardiac ultrasound (US) image segmentation is vital for evaluating clinical indices, but it often demands a large dataset and expert annotations, resulting in high costs for deep learning algorithms. To address this, our study presents a framework utilizing artificial intelligence generation technology to produce multi-class RGB masks for cardiac US image segmentation. The proposed approach directly performs semantic segmentation of the heart's main structures in US images from various scanning modes. Additionally, we introduce a novel learning approach based on conditional generative adversarial networks (CGAN) for cardiac US image segmentation, incorporating a conditional input and paired RGB masks. Experimental results from three cardiac US image datasets with diverse scan modes demonstrate that our approach outperforms several state-of-the-art models, showcasing improvements in five commonly used segmentation metrics, with lower noise sensitivity. Source code is available at https://github.com/energy588/US2mask., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The development of automated methods for analyzing medical images of colon cancer is one of the main research fields. A colonoscopy is a medical treatment that enables a doctor to look for any abnormalities like polyps, cancer, or inflammatory tissue inside the colon and rectum. It falls under the category of gastrointestinal illnesses, and it claims the lives of almost two million people worldwide. Video endoscopy is an advanced medical imaging approach to diagnose gastrointestinal disorders such as inflammatory bowel, ulcerative colitis, esophagitis, and polyps. Medical video endoscopy generates several images, which must be reviewed by specialists. The difficulty of manual diagnosis has sparked research towards computer-aided techniques that can quickly and reliably diagnose all generated images. The proposed methodology establishes a framework for diagnosing coloscopy diseases. Endoscopists can lower the risk of polyps turning into cancer during colonoscopies by using more accurate computer-assisted polyp detection and segmentation. With the aim of creating a model that can automatically distinguish polyps from images, we presented a modified DeeplabV3+ model in this study to carry out segmentation tasks successfully and efficiently. The framework's encoder uses a pre-trained dilated convolutional residual network for optimal feature map resolution. The robustness of the modified model is tested against state-of-the-art segmentation approaches. In this work, we employed two publicly available datasets, CVC-Clinic DB and Kvasir-SEG, and obtained Dice similarity coefficients of 0.97 and 0.95, respectively. The results show that the improved DeeplabV3+ model improves segmentation efficiency and effectiveness in both software and hardware with only minor changes., Competing Interests: Declaration of competing interest The authors declare no conflict of interests. There is no financial help from any organization., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Recently, Large Language Models (LLMs) have demonstrated impressive capability to solve a wide range of tasks. However, despite their success across various tasks, no prior work has investigated their capability in the biomedical domain yet. To this end, this paper aims to evaluate the performance of LLMs on benchmark biomedical tasks. For this purpose, a comprehensive evaluation of 4 popular LLMs in 6 diverse biomedical tasks across 26 datasets has been conducted. To the best of our knowledge, this is the first work that conducts an extensive evaluation and comparison of various LLMs in the biomedical domain. Interestingly, we find based on our evaluation that in biomedical datasets that have smaller training sets, zero-shot LLMs even outperform the current state-of-the-art models when they were fine-tuned only on the training set of these datasets. This suggests that pre-training on large text corpora makes LLMs quite specialized even in the biomedical domain. We also find that not a single LLM can outperform other LLMs in all tasks, with the performance of different LLMs may vary depending on the task. While their performance is still quite poor in comparison to the biomedical models that were fine-tuned on large training sets, our findings demonstrate that LLMs have the potential to be a valuable tool for various biomedical tasks that lack large annotated data., Competing Interests: Declaration of competing interest The authors declare having no competing interests., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Background: Segmenting colorectal polyps presents a significant challenge due to the diverse variations in their size, shape, texture, and intricate backgrounds. Particularly demanding are the so-called "camouflaged" polyps, which are partially concealed by surrounding tissues or fluids, adding complexity to their detection., Methods: We present CPSNet, an innovative model designed for camouflaged polyp segmentation. CPSNet incorporates three key modules: the Deep Multi-Scale-Feature Fusion Module, the Camouflaged Object Detection Module, and the Multi-Scale Feature Enhancement Module. These modules work collaboratively to improve the segmentation process, enhancing both robustness and accuracy., Results: Our experiments confirm the effectiveness of CPSNet. When compared to state-of-the-art methods in colon polyp segmentation, CPSNet consistently outperforms the competition. Particularly noteworthy is its performance on the ETIS-LaribPolypDB dataset, where CPSNet achieved a remarkable 2.3% increase in the Dice coefficient compared to the Polyp-PVT model., Conclusion: In summary, CPSNet marks a significant advancement in the field of colorectal polyp segmentation. Its innovative approach, encompassing multi-scale feature fusion, camouflaged object detection, and feature enhancement, holds considerable promise for clinical applications., Competing Interests: Declaration of competing interest The authors of this submission to ‘Computers in Biology and Medicine’ have no financial interests or personal relationships that could be perceived to have influenced the research., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The worldwide COVID-19 pandemic has profoundly influenced the health and everyday experiences of individuals across the planet. It is a highly contagious respiratory disease requiring early and accurate detection to curb its rapid transmission. Initial testing methods primarily revolved around identifying the genetic composition of the coronavirus, exhibiting a relatively low detection rate and requiring a time-intensive procedure. To address this challenge, experts have suggested using radiological imagery, particularly chest X-rays, as a valuable approach within the diagnostic protocol. This study investigates the potential of leveraging radiographic imaging (X-rays) with deep learning algorithms to swiftly and precisely identify COVID-19 patients. The proposed approach elevates the detection accuracy by fine-tuning with appropriate layers on various established transfer learning models. The experimentation was conducted on a COVID-19 X-ray dataset containing 2000 images. The accuracy rates achieved were impressive of 99.55%, 97.32%, 99.11%, 99.55%, 99.11% and 100% for Xception, InceptionResNetV2, ResNet50 , ResNet50V2, EfficientNetB0 and EfficientNetB4 respectively. The fine-tuned EfficientNetB4 achieved an excellent accuracy score, showcasing its potential as a robust COVID-19 detection model. Furthermore, EfficientNetB4 excelled in identifying Lung disease using Chest X-ray dataset containing 4,350 Images, achieving remarkable performance with an accuracy of 99.17%, precision of 99.13%, recall of 99.16%, and f1-score of 99.14%. These results highlight the promise of fine-tuned transfer learning for efficient lung detection through medical imaging, especially with X-ray images. This research offers radiologists an effective means of aiding rapid and precise COVID-19 diagnosis and contributes valuable assistance for healthcare professionals in accurately identifying affected patients., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

To address the scarcity and class imbalance of abnormal electrocardiogram (ECG) databases, which are crucial in AI-driven diagnostic tools for potential cardiovascular disease detection, this study proposes a novel quantum conditional generative adversarial algorithm (QCGAN-ECG) for generating abnormal ECG signals. The QCGAN-ECG constructs a quantum generator based on patch method. In this method, each sub-generator generates distinct features of abnormal heartbeats in different segments. This patch-based generative algorithm conserves quantum resources and makes QCGAN-ECG practical for near-term quantum devices. Additionally, QCGAN-ECG introduces quantum registers as control conditions. It encodes information about the types and probability distributions of abnormal heartbeats into quantum registers, rendering the entire generative process controllable. Simulation experiments on Pennylane demonstrated that the QCGAN-ECG could generate completely abnormal heartbeats with an average accuracy of 88.8%. Moreover, the QCGAN-ECG can accurately fit the probability distribution of various abnormal ECG data. In the anti-noise experiments, the QCGAN-ECG showcased outstanding robustness across various levels of quantum noise interference. These results demonstrate the effectiveness and potential applicability of the QCGAN-ECG for generating abnormal ECG signals, which will further promote the development of AI-driven cardiac disease diagnosis systems. The source code is available at github.com/VanSWK/QCGAN_ECG., Competing Interests: Declaration of competing interest The authors declare no conflict of interests., (Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

During the last decade, genomic, transcriptomic, proteomic, metabolomic, and other omics datasets have been generated for a wide range of marine organisms, and even more are still on the way. Marine organisms possess unique and diverse biosynthetic pathways contributing to the synthesis of novel secondary metabolites with significant bioactivities. As marine organisms have a greater tendency to adapt to stressed environmental conditions, the chance to identify novel bioactive metabolites with potential biotechnological application is very high. This review presents a comprehensive overview of the available "-omics" and "multi-omics" approaches employed for characterizing marine metabolites along with novel data integration tools. The need for the development of machine-learning algorithms for "multi-omics" approaches is briefly discussed. In addition, the challenges involved in the analysis of "multi-omics" data and recommendations for conducting "multi-omics" study were discussed., Competing Interests: Declaration of competing interest On behalf of all authors, I as corresponding author declare that none of the authors has a conflict of interest., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Social media platforms such as Twitter are home ground for rapid COVID-19-related information sharing over the Internet, thereby becoming the favorable data resource for many downstream applications. Due to the massive pile of COVID-19 tweets generated every day, it is significant that the machine-learning-supported downstream applications can effectively skip the uninformative tweets and only pick up the informative tweets for their further use. However, existing solutions do not specifically consider the negative effect caused by the imbalanced ratios between informative and uninformative tweets in training data. In particular, most of the existing solutions are dominated by single-view learning, neglecting the rich information from different views to facilitate learning. In this study, a novel deep imbalanced multi-view learning approach called D-SVM-2K is proposed to identify the informative COVID-19 tweets from social media. This approach is built upon the well-known multiview learning method SVM-2K to incorporate different views generated from different feature extraction techniques. To battle against the class imbalance problem and enhance its learning ability, D-SVM-2K stacks multiple SVM-2K base classifiers in a stacked deep structure where its base classifiers can learn from either the original training dataset or the shifted critical regions identified using the well-known k-nearest neighboring algorithm. D-SVM-2K also realises a global and local deep ensemble learning on the multiple views' data. Our empirical experiments on a real-world labeled tweet dataset demonstrate the effectiveness of D-SVM-2K in dealing with the real-world multi-view class imbalance issues., Competing Interests: Declaration of competing interest None Declared., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Performing prescribed physical exercises during home-based rehabilitation programs plays an important role in regaining muscle strength and improving balance for people with different physical disabilities. However, patients attending these programs are not able to assess their action performance in the absence of a medical expert. Recently, vision-based sensors have been deployed in the activity monitoring domain. They are capable of capturing accurate skeleton data. Furthermore, there have been significant advancements in Computer Vision (CV) and Deep Learning (DL) methodologies. These factors have promoted the solutions for designing automatic patient's activity monitoring models. Then, improving such systems' performance to assist patients and physiotherapists has attracted wide interest of the research community. This paper provides a comprehensive and up-to-date literature review on different stages of skeleton data acquisition processes for the aim of physio exercise monitoring. Then, the previously reported Artificial Intelligence (AI) - based methodologies for skeleton data analysis will be reviewed. In particular, feature learning from skeleton data, evaluation, and feedback generation for the purpose of rehabilitation monitoring will be studied. Furthermore, the associated challenges to these processes will be reviewed. Finally, the paper puts forward several suggestions for future research directions in this area., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

In this paper, a Covid-19 dynamical transmission model of a coupled non-linear fractional differential equation in the Atangana-Baleanu Caputo sense is proposed. The basic dynamical transmission features of the proposed system are briefly discussed. The qualitative as well as quantitative results on the existence and uniqueness of the solutions are evaluated through the fixed point theorem. The Ulam-Hyers stability analysis of the suggested system is established. The two-step Adams-Bashforth-Moulton (ABM) numerical method is employed to find its numerical solution. The numerical simulation is performed to accesses the impact of various biological parameters on the dynamics of Covid-19 disease., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

DNA-binding proteins (DBPs) protect DNA from nuclease hydrolysis, inhibit the action of RNA polymerase, prevents replication and transcription from occurring simultaneously on a piece of DNA. Most of the conventional methods for detecting DBPs are biochemical methods, but the time cost is high. In recent years, a variety of machine learning-based methods that have been used on a large scale for large-scale screening of DBPs. To improve the prediction performance of DBPs, we propose a random Fourier features-based sparse representation classifier (RFF-SRC), which randomly map the features into a high-dimensional space to solve nonlinear classification problems. And L 2,1 -matrix norm is introduced to get sparse solution of model. To evaluate performance, our model is tested on several benchmark data sets of DBPs and 8 UCI data sets. RFF-SRC achieves better performance in experimental results., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

In recent years, the global Internet of Medical Things (IoMT) industry has evolved at a tremendous speed. Security and privacy are key concerns on the IoMT, owing to the huge scale and deployment of IoMT networks. Machine learning (ML) and blockchain (BC) technologies have significantly enhanced the capabilities and facilities of healthcare 5.0, spawning a new area known as "Smart Healthcare." By identifying concerns early, a smart healthcare system can help avoid long-term damage. This will enhance the quality of life for patients while reducing their stress and healthcare costs. The IoMT enables a range of functionalities in the field of information technology, one of which is smart and interactive health care. However, combining medical data into a single storage location to train a powerful machine learning model raises concerns about privacy, ownership, and compliance with greater concentration. Federated learning (FL) overcomes the preceding difficulties by utilizing a centralized aggregate server to disseminate a global learning model. Simultaneously, the local participant keeps control of patient information, assuring data confidentiality and security. This article conducts a comprehensive analysis of the findings on blockchain technology entangled with federated learning in healthcare. 5.0. The purpose of this study is to construct a secure health monitoring system in healthcare 5.0 by utilizing a blockchain technology and Intrusion Detection System (IDS) to detect any malicious activity in a healthcare network and enables physicians to monitor patients through medical sensors and take necessary measures periodically by predicting diseases. The proposed system demonstrates that the approach is optimized effectively for healthcare monitoring. In contrast, the proposed healthcare 5.0 system entangled with FL Approach achieves 93.22% accuracy for disease prediction, and the proposed RTS-DELM-based secure healthcare 5.0 system achieves 96.18% accuracy for the estimation of intrusion detection., Competing Interests: Declaration of competing interest It declares there is no conflict of interest., (Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)

The emergence of large-scale human genome projects, advances in DNA sequencing technologies, and the massive volume of electronic medical records [EMR] shift the transformation of healthcare research into the next paradigm, namely 'Precision Medicine.' This new clinical system model uses patients' genomic profiles and disparate healthcare data sources to a greater extent and provides personalized deliverables. As an advanced analytical technique, deep learning models significantly impact precision medicine because they can process voluminous amounts of diversified data with improved accuracy. Two salient features of deep learning models, namely processing a massive volume of multi-model data at multiple levels of abstraction and the ability to identify inherent features from the input data on their own, attract the implication of deep learning techniques in precision medicine research. The proposed review highlights the importance of deep learning-based analytical models in handling diversified and disparate big data sources of precision medicine. To augment further, state-of-the-art precision medicine research based on the taxonomy of deep learning models has been reviewed along with their research outcomes. The diversified data inputs used in research attempts, their applications, benchmarking data repositories, and usage of various evaluation measures for accuracy estimations are highlighted in this review. This review also brings out some promising analytical avenues of precision medicine research that give directions for future exploration., Competing Interests: Declaration of competing interest The authors declare that they have no conflicts of interest., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Histopathological image analysis is the gold standard to diagnose cancer. Carcinoma is a subtype of cancer that constitutes more than 80% of all cancer cases. Squamous cell carcinoma and adenocarcinoma are two major subtypes of carcinoma, diagnosed by microscopic study of biopsy slides. However, manual microscopic evaluation is a subjective and time-consuming process. Many researchers have reported methods to automate carcinoma detection and classification. The increasing use of artificial intelligence (AI) in the automation of carcinoma diagnosis also reveals a significant rise in the use of deep network models. In this systematic literature review, we present a comprehensive review of the state-of-the-art approaches reported in carcinoma diagnosis using histopathological images. Studies are selected from well-known databases with strict inclusion/exclusion criteria. We have categorized the articles and recapitulated their methods based on specific organs of carcinoma origin. Further, we have summarized pertinent literature on AI methods, highlighted critical challenges and limitations, and provided insights on future research direction in automated carcinoma diagnosis. Out of 101 articles selected, most of the studies experimented on private datasets with varied image sizes, obtaining accuracy between 63% and 100%. Overall, this review highlights the need for a generalized AI-based carcinoma diagnostic system. Additionally, it is desirable to have accountable approaches to extract microscopic features from images of multiple magnifications that should mimic pathologists' evaluations., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Ageing is associated with various ailments including Alzheimer 's disease (AD), which is a progressive form of dementia. AD symptoms develop over a period of years and, unfortunately, there is no cure. Existing AD treatments can only slow down the progression of symptoms and thus it is critical to diagnose the disease at an early stage. To help improve the early diagnosis of AD, a deep learning-based classification model with an embedded feature selection approach was used to classify AD patients. An AD DNA methylation data set (64 records with 34 cases and 34 controls) from the GEO omnibus database was used for the analysis. Before selecting the relevant features, the data were preprocessed by performing quality control, normalization and downstream analysis. As the number of associated CpG sites was huge, four embedded-based feature selection models were compared and the best method was used for the proposed classification model. An Enhanced Deep Recurrent Neural Network (EDRNN) was implemented and compared to other existing classification models, including a Convolutional Neural Network (CNN), a Recurrent Neural Network (RNN), and a Deep Recurrent Neural Network (DRNN). The results showed a significant improvement in the classification accuracy of the proposed model as compared to the other methods., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Medical image acquisition plays a significant role in the diagnosis and management of diseases. Magnetic Resonance (MR) and Computed Tomography (CT) are considered two of the most popular modalities for medical image acquisition. Some considerations, such as cost and radiation dose, may limit the acquisition of certain image modalities. Therefore, medical image synthesis can be used to generate required medical images without actual acquisition. In this paper, we propose a paired-unpaired Unsupervised Attention Guided Generative Adversarial Network (uagGAN) model to translate MR images to CT images and vice versa. The uagGAN model is pre-trained with a paired dataset for initialization and then retrained on an unpaired dataset using a cascading process. In the paired pre-training stage, we enhance the loss function of our model by combining the Wasserstein GAN adversarial loss function with a new combination of non-adversarial losses (content loss and L1) to generate fine structure images. This will ensure global consistency, and better capture of the high and low frequency details of the generated images. The uagGAN model is employed as it generates more accurate and sharper images through the production of attention masks. Knowledge from a non-medical pre-trained model is also transferred to the uagGAN model for improved learning and better image translation performance. Quantitative evaluation and qualitative perceptual analysis by radiologists indicate that employing transfer learning with the proposed paired-unpaired uagGAN model can achieve better performance as compared to other rival image-to-image translation models., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Head computed tomography (CT) is the gold standard in emergency departments (EDs) to evaluate mild traumatic brain injury (mTBI) patients, especially for paediatrics. Data-driven models for successfully classifying head CT scans that have mTBI will be valuable in terms of timeliness and cost-effectiveness for TBI diagnosis. This study applied two different machine learning (ML) models to diagnose mTBI in a paediatric population collected as part of the paediatric emergency care applied research network (PECARN) study between 2004 and 2006. The models were conducted using 15,271 patients under the age of 18 years with mTBI and had a head CT report. In the conventional model, random forest (RF) ranked the features to reduce data dimensionality and the top ranked features were used to train a shallow artificial neural network (ANN) model. In the second model, a deep ANN applied to classify positive and negative mTBI patients using the entirety of the features available. The dataset was divided into two subsets: 80% for training and 20% for testing using five-fold cross-validation. Accuracy, sensitivity, precision, and specificity were calculated by comparing the model's prediction outcome to the actual diagnosis for each patient. RF ranked ten clinical demographic features and twelve CT-findings; the hybrid RF-ANN model achieved an average specificity of 99.96%, sensitivity of 95.98%, precision of 99.25%, and accuracy of 99.74% in identifying positive mTBI from negative mTBI subjects. The deep ANN proved its ability to carry out the task efficiently with an average specificity of 99.9%, sensitivity of 99.2%, precision of 99.9%, and accuracy of 99.9%. The performance of the two proposed models demonstrated the feasibility of using ANN to diagnose mTBI in a paediatric population. This is the first study to investigate deep ANN in a paediatric cohort with mTBI using clinical and non-imaging data and diagnose mTBI with balanced sensitivity and specificity using shallow and deep ML models. This method, if validated, would have the potential to reduce the burden of TBI evaluation in EDs and aide clinicians in the decision-making process., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

A deep learning pipeline was developed and used to localize and classify a variety of implants in the femur contained in whole-body post-mortem computed tomography (PMCT) scans. The results provide a proof-of-principle approach for labelling content not described in medical/autopsy reports. The pipeline, which incorporated residual networks and an autoencoder, was trained and tested using n = 450 full-body PMCT scans. For the localization component, Dice scores of 0.99, 0.96, and 0.98 and mean absolute errors of 3.2, 7.1, and 4.2 mm were obtained in the axial, coronal, and sagittal views, respectively. A regression analysis found the orientation of the implant to the scanner axis and also the relative positioning of extremities to be statistically significant factors. For the classification component, test cases were properly labelled as nail (N + ), hip replacement (H + ), knee replacement (K + ) or without-implant (I - ) with an accuracy >97%. The recall for I - and H + cases was 1.00, but fell to 0.82 and 0.65 for cases with K + and N + . This semi-automatic approach provides a generalized structure for image-based labelling of features, without requiring time-consuming segmentation., Competing Interests: Declaration of competing interest The authors certify that there is no conflict of interest with any financial organization regarding the materials presented in the manuscript., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Phonocardiogram (PCG) signals reflect the mechanical activity of the heart. Previous studies have reported that PCG signals contain heart murmurs caused by coronary artery disease (CAD). However, the murmurs caused by CAD are very weak and rarely heard by the human ear. In this paper, a novel feature fusion framework is proposed to provide a comprehensive basis for CAD diagnosis. A dataset containing PCG signals of 175 subjects was collected and used. A total of 110 features were extracted from multiple domains, and then reduced and selected. Images obtained by Mel-frequency cepstral coefficients were used as the input for the convolutional neural network for feature learning. Then, the selected features and the deep learning features were fused and fed into a multilayer perceptron for classification. The proposed feature fusion method achieved better classification performance than multi-domain features or deep learning features alone, with accuracy, sensitivity, and specificity of 90.43%, 93.67%, and 83.36%, respectively. A comparison with existing studies demonstrated that the proposed method was a promising noninvasive screening tool for CAD under general medical conditions., Competing Interests: Declaration of competing interest We declare that we have no conflicts of interest., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Numerous automatic epileptic seizure detectors (ESDs) with excellent performances have been reported, but they generally experience performance degradation when tested with real-life clinical data. This has been blamed on the scarcity of high-quality training data, which leads to models that generalize poorly. There is consequently interest in methods to improve the quality and quantity of training data for ESDs. This study used a domain generalization approach to combine data from two different datasets for training an ESD, which was thereafter tested on a third dataset. A subspace of the CHB-MIT and TUSZ scalp EEG seizure datasets was extracted using transfer component analysis, based on a reproducing kernel Hilbert space approach. We then used the Azimuthal Equidistant Projection to transform 3D electrode coordinates into 2D space, followed by interpolation using the Clough-Tocher technique to generate 16x16 rasters. We thereafter generated feature vectors, each of which was a sequence of 17 ten-layer 16x16 raster arrays. The vectors were used to train a recurrent-convolutional neural network. The network had a 128-unit long short-term memory layer with inputs from 17 parallel networks each with three stacks of convolutional layers. Testing was based on a private 26-subject dataset, combined with randomly selected subsets of the CHB-MIT and TUSZ datasets. A combined sensitivity of 74.5% was achieved, along with a false positive per hour rate of 0.84, and a latency of 2.32 s. Detection sensitivity on the private dataset was 72.5%. These results compare favorably with results of large-scale validation studies in literature and confirm the viability of this approach to increasing the size of training datasets for ESDs., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Sub-clinical bovine mastitis decreases milk quality and production. Moreover, sub-clinical mastitis leads to the use of antibiotics with consequent increased risk of the emergence of antibiotic-resistant bacteria. Therefore, early detection of infected cows is of great importance. The Somatic Cell Count (SCC) day-test used for mastitis surveillance, gives data that fluctuate widely between days, creating questions about its reliability and early prediction power. The recent identification of risk parameters of sub-clinical mastitis based on milking parameters by machine learning models is emerging as a promising new tool to enhance early prediction of mastitis occurrence. To develop the optimal approach for early sub-clinical mastitis prediction, we implemented 2 steps: (1) Finding the best statistical models to accurately link patterns of risk factors to sub-clinical mastitis, and (2) Extending this application from the farms tested to new farms (method generalization). Herein, we applied various machine learning-based prediction systems on a big milking dataset to uncover the best predictive models of sub-clinical mastitis. Data from 364,249 milking instances were collected by an electronic automated in-line monitoring system where milk volume, lactose concentration, electrical conductivity (EC), protein concentration, peak flow and milking time for each sample were measured. To provide a platform for the application of the models developed to other farms, the Z transformation approach was employed. Following this, various prediction systems [Deep Learning (DL), Naïve Bayes, Generalized Liner Model, Logistic Regression, Decision Tree, Gradient-Boosted Tree (GBT) and Random Forest] were applied to the non-transformed milking dataset and to a Z-standardized dataset. ROC (Receiver Operating Characteristics Curve), AUC (Area Under The Curve), and high accuracy demonstrated the high sensitivity of GBT and DL in detecting sub-clinical mastitis. GBT was the most accurate model (accuracy of 84.9%) in prediction of sub-clinical bovine mastitis. These data demonstrate how these models could be applied for prediction of sub-clinical mastitis in multiple bovine herds regardless of the size and sampling techniques., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

The Gene or DNA sequence in every cell does not control genetic properties on its own; Rather, this is done through the translation of DNA into protein and subsequent formation of a certain 3D structure. The biological function of a protein is tightly connected to its specific 3D structure. Prediction of the protein secondary structure is a crucial intermediate step towards elucidating its 3D structure and function. Traditional experimental methods for prediction of protein structure are expensive and time-consuming. Nevertheless, the average accuracy of the suggested solutions has hardly reached beyond 80%. The possible underlying reasons are the ambiguous sequence-structure relation, noise in input protein data, class imbalance, and the high dimensionality of the encoding schemes. Furthermore, we utilize a compound string dissimilarity measure to directly interpret protein sequence content and avoid information loss. In order to improve accuracy, we employ two different classifiers including support vector machine and fuzzy nearest neighbor and collectively aggregate the classification outcomes to infer the final protein structures. We conduct comprehensive experiments to compare our model with the current state-of-the-art approaches. The experimental results demonstrate that given a set of input sequences, our multi-component framework can accurately predict the protein structure. Nevertheless, the effectiveness of our unified model can be further enhanced through framework configuration., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Objective: Research was conducted on parametric profiles of healthy subjects and patients with cervico-brachial pain syndrome resulting from C4/5 and/or C5/6 discopathy, including magnetic remanence of tissues in marker points 1-12 (L+R) and functional parameters, and their subsequent change after treatment in group A, using method of push-pull galvanic magnetostimulation (GMT 2.0)., Design: GMT 2.0 device, comprised of one air solenoid and three galvanic solenoids in electrolytic tubs, was designed for push-pull magnetostimulation of the head, coupled with simultaneous stimulation of the limbs., Setting: Clinical trial was conducted in Outpatient Private Clinic "VIS" under the auspices of Silesian Higher Medical School in Katowice, Poland., Patients: 55 subjects participated in the study: control group K consisted of 23 healthy individuals, whereas 33 patients in group A were treated using GMT 2.0., Intervention: Only patients in group A were treated with GMT 2.0 during 40-min sessions over a period of 10 days., Main Outcome Measures: Parametric profile of the patients was defined using various measurements: electronic SFTR test (C-Th-shoulders), HR, RR, BDI and VAS tests, magnetic remanence in marker points 1-12 (L+R) and blood parameters: HB, ER, CREA, BIL, K(+), Na(+), Cl(-) Fe(2+), Ca(2+) and Mg(2+)., Results: There was a significant reduction in pain (VAS), increase in the range of motion (SFTR), lower depression symptoms (BDI), slower heart rate (HR), lower blood pressure (RR), greater concentration of Mg(2+), K(+), Ca(2+)ions and reduction in the concentration of BIL, CREA Fe(2+) after GMT 2.0 treatment in group A. Evaluation of magnetic remanence in marker points M1-12 (L+R) initially showed higher values in group K, which after treatment were normalized to values similar to those in group K., Conclusion: GMT 2.0 treatment in group A resulted in normalization of magnetic remanence, synergically with increased range of motion (SFTR test), decreased HR and RR parameters, smaller depressive trends (BDI test), as well as increased ion levels (K(+), Mg(2+), Ca(2+)) and better functional parameters of kidneys and liver., (Copyright © 2015 Elsevier Ltd. All rights reserved.)

MicroRNAs are small, endogenous RNAs found in many different species and are known to have an influence on diverse biological phenomena. They also play crucial roles in plant biological processes, such as metabolism, leaf sidedness and flower development. However, the functional roles of most microRNAs are still unknown. The identification of closely related microRNAs and target genes can be an essential first step towards the discovery of their combinatorial effects on different cellular states. A lot of research has tried to discover microRNAs and target gene interactions by implementing machine learning classifiers with target prediction algorithms. However, high rates of false positives have been reported as a result of undetermined factors which will affect recognition. Therefore, integrating diverse techniques could improve the prediction. In this paper we propose identifying microRNAs target of Arabidopsis thaliana by integrating prediction scores from PITA, miRanda and RNAHybrid algorithms used as a feature vector of microRNA-target interactions, and then implementing SVM, random forest tree and neural network machine learning algorithms to make final predictions by majority voting. Furthermore, microRNA target genes are linked with their protein-protein interaction (PPI) partners. We focus on plant resistance genes and transcription factor information to provide new insights into plant pathogen interaction networks. Downstream pathways are characterized by the Jaccard coefficient, which is implemented based on Gene Ontology. The database is freely accessible at http://ppi.bioinfo.asia.edu.tw/At_miRNA/., (Copyright © 2013 Elsevier Ltd. All rights reserved.)

Cardiovascular autonomic neuropathy (CAN) is a serious and well known complication of diabetes. Previous articles circumvented the problem of missing values in CAN data by deleting all records and fields with missing values and applying classifiers trained on different sets of features that were complete. Most of them also added alternative features to compensate for the deleted ones. Here we introduce and investigate a new method for classifying CAN data with missing values. In contrast to all previous papers, our new method does not delete attributes with missing values, does not use classifiers, and does not add features. Instead it is based on regression and meta-regression combined with the Ewing formula for identifying the classes of CAN. This is the first article using the Ewing formula and regression to classify CAN. We carried out extensive experiments to determine the best combination of regression and meta-regression techniques for classifying CAN data with missing values. The best outcomes have been obtained by the additive regression meta-learner based on M5Rules and combined with the Ewing formula. It has achieved the best accuracy of 99.78% for two classes of CAN, and 98.98% for three classes of CAN. These outcomes are substantially better than previous results obtained in the literature by deleting all missing attributes and applying traditional classifiers to different sets of features without regression. Another advantage of our method is that it does not require practitioners to perform more tests collecting additional alternative features., (© 2013 Elsevier Ltd. All rights reserved.)

Breathing dynamics vary between infant sleep states, and are likely to exhibit non-linear behaviour. This study applied the non-linear analytical tool recurrence quantification analysis (RQA) to 400 breath interval periods of REM and N-REM sleep, and then using an overlapping moving window. The RQA variables were different between sleep states, with REM radius 150% greater than N-REM radius, and REM laminarity 79% greater than N-REM laminarity. RQA allowed the observation of temporal variations in non-linear breathing dynamics across a night's sleep at 30s resolution, and provides a basis for quantifying changes in complex breathing dynamics with physiology and pathology., (Copyright © 2013 Elsevier Ltd. All rights reserved.)

This paper presents image and video analysis based schemes to automate the process of adductors angle measurement which is carried out on infants as a part of Hammersmith Infant Neurological Examination (HINE). Image segmentation, thinning and feature point based object tracking are used for automating the analysis. Segmentation outputs are processed with a novel region merging algorithm. It is found that the refined segmentation outputs can successfully be used to extract features in the context of the application under consideration. Next, a heuristic based filtering algorithm is applied on the thinned structures for locating necessary points to measure adductors angle. A semi-automatic scheme based on the object tracking of a video has been proposed to minimize errors of the image based analysis. It is observed that the video-based analysis outperforms the image-based method. A fully automatic method has also been proposed and compared with the semi-automatic algorithm. The proposed methods have been tested with several videos recorded from hospitals and the results have been found to be satisfactory in the present context., (Copyright © 2012 Elsevier Ltd. All rights reserved.)